Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1565822..1566384 Replicon chromosome
Accession NZ_CP095526
Organism Escherichia coli strain GN05505

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag MW395_RS08235 Protein ID WP_000605676.1
Coordinates 1565822..1566100 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag MW395_RS08240 Protein ID WP_000781370.1
Coordinates 1566100..1566384 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW395_RS08210 (1561408) 1561408..1561839 - 432 WP_000152305.1 peroxiredoxin OsmC -
MW395_RS08215 (1562184) 1562184..1562399 + 216 WP_000495766.1 biofilm-dependent modulation protein -
MW395_RS08220 (1562501) 1562501..1562638 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
MW395_RS08225 (1562795) 1562795..1564492 + 1698 WP_000433464.1 malate dehydrogenase -
MW395_RS08230 (1564626) 1564626..1565636 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
MW395_RS08235 (1565822) 1565822..1566100 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MW395_RS08240 (1566100) 1566100..1566384 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
MW395_RS08245 (1566612) 1566612..1567265 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
MW395_RS08250 (1567258) 1567258..1568142 - 885 WP_001240565.1 formate dehydrogenase N subunit beta -
MW395_RS08255 (1568155) 1568155..1571202 - 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T242257 WP_000605676.1 NZ_CP095526:1565822-1566100 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T242257 NZ_OW052302:128349-128678 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT242257 WP_000781370.1 NZ_CP095526:1566100-1566384 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT242257 NZ_OW052302:127975-128352 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References