Detailed information of TA system TA242241

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 14288..14557
• Replicon: plasmid p4171-2
• Accession: NZ_CP095524
• Organism: Escherichia coli strain GN04171

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MW404_RS24995
• Protein ID: WP_001372321.1
• Coordinates: 14432..14557 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	14288..14353 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW404_RS24955	9324..9755	-	432	Protein_15	hypothetical protein	-
MW404_RS24960	10057..10584	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
MW404_RS24965	10642..10875	+	234	WP_000006003.1	DUF905 family protein	-
MW404_RS24970	10936..12900	+	1965	WP_000117316.1	ParB/RepB/Spo0J family partition protein	-
MW404_RS24975	12969..13403	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
MW404_RS24980	13400..14162	+	763	Protein_20	plasmid SOS inhibition protein A	-
-	14131..14355	+	225	NuclAT_0	-	-
-	14131..14355	+	225	NuclAT_0	-	-
-	14131..14355	+	225	NuclAT_0	-	-
-	14131..14355	+	225	NuclAT_0	-	-
MW404_RS24985	14140..14319	-	180	WP_001309233.1	hypothetical protein	-
-	14288..14353	-	66	-	-	Antitoxin
MW404_RS24990	14341..14490	+	150	Protein_22	plasmid maintenance protein Mok	-
MW404_RS24995	14432..14557	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
MW404_RS25000	14876..15173	-	298	Protein_24	hypothetical protein	-
MW404_RS25005	15473..15769	+	297	WP_001272251.1	hypothetical protein	-
MW404_RS25010	15880..16701	+	822	WP_001234445.1	DUF932 domain-containing protein	-
MW404_RS25015	16998..17600	-	603	Protein_27	transglycosylase SLT domain-containing protein	-
MW404_RS25020	17921..18304	+	384	WP_001151538.1	conjugal transfer relaxosome DNA-binding protein TraM	-
MW404_RS25025	18491..19180	+	690	WP_016239105.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..74318	74318

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T242241 WP_001372321.1 NZ_CP095524:14432-14557 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T242241 NZ_OW052189:c4028607-4028278 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT242241 NZ_CP095524:c14353-14288 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin