Detailed information of TA system TA242237

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 67043..67282
• Replicon: plasmid p4171-3
• Accession: NZ_CP095522
• Organism: Escherichia coli strain GN04171

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: MW404_RS24825
• Protein ID: WP_023144756.1
• Coordinates: 67043..67177 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	67222..67282 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW404_RS24790 (63053)	63053..63454	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
MW404_RS24795 (63387)	63387..63644	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
MW404_RS24800 (63737)	63737..64390	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
MW404_RS24805 (65331)	65331..66188	-	858	WP_162758310.1	incFII family plasmid replication initiator RepA	-
MW404_RS24810 (66181)	66181..66255	-	75	WP_031943482.1	RepA leader peptide Tap	-
MW404_RS24815 (66255)	66255..66386	-	132	Protein_88	protein CopA/IncA	-
MW404_RS24820 (66492)	66492..66746	-	255	WP_000083850.1	replication regulatory protein RepA	-
MW404_RS24825 (67043)	67043..67177	-	135	WP_023144756.1	Hok/Gef family protein	Toxin
- (67222)	67222..67282	+	61	NuclAT_2	-	Antitoxin
- (67222)	67222..67282	+	61	NuclAT_2	-	Antitoxin
- (67222)	67222..67282	+	61	NuclAT_2	-	Antitoxin
- (67222)	67222..67282	+	61	NuclAT_2	-	Antitoxin
MW404_RS24830 (67249)	67249..67535	-	287	Protein_91	DUF2726 domain-containing protein	-
MW404_RS24835 (67613)	67613..67887	-	275	WP_141072115.1	transposase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B	-	1..67887	67887
	-	flank	IS/Tn	-	-	67613..67798	185

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T242237 WP_023144756.1 NZ_CP095522:c67177-67043 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T242237 NZ_OW052189:3537153-3537470 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT242237 NZ_CP095522:67222-67282 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin