Detailed information of TA system TA242232

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 19293..19535
• Replicon: plasmid p4171-3
• Accession: NZ_CP095522
• Organism: Escherichia coli strain GN04171

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MW404_RS24515
• Protein ID: WP_001372321.1
• Coordinates: 19293..19418 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	19495..19535 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW404_RS24470 (14405)	14405..14632	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
MW404_RS24475 (14720)	14720..15397	-	678	WP_001348626.1	PAS domain-containing protein	-
MW404_RS24480 (15531)	15531..15914	-	384	WP_001151566.1	conjugal transfer relaxosome DNA-binding protein TraM	-
MW404_RS24485 (16245)	16245..16847	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
MW404_RS24490 (17144)	17144..17965	-	822	WP_001234469.1	DUF932 domain-containing protein	-
MW404_RS24495 (18084)	18084..18371	-	288	WP_000107535.1	hypothetical protein	-
MW404_RS24500 (18396)	18396..18602	-	207	WP_024261895.1	hypothetical protein	-
MW404_RS24505 (18672)	18672..18845	+	174	Protein_26	hypothetical protein	-
MW404_RS24510 (18843)	18843..19073	-	231	WP_001426396.1	hypothetical protein	-
MW404_RS24515 (19293)	19293..19418	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
MW404_RS24520 (19360)	19360..19509	-	150	Protein_29	plasmid maintenance protein Mok	-
- (19495)	19495..19535	-	41	NuclAT_1	-	Antitoxin
- (19495)	19495..19535	-	41	NuclAT_1	-	Antitoxin
- (19495)	19495..19535	-	41	NuclAT_1	-	Antitoxin
- (19495)	19495..19535	-	41	NuclAT_1	-	Antitoxin
- (20979)	20979..21165	-	187	NuclAT_0	-	-
- (20979)	20979..21165	-	187	NuclAT_0	-	-
- (20979)	20979..21165	-	187	NuclAT_0	-	-
- (20979)	20979..21165	-	187	NuclAT_0	-	-
MW404_RS24530 (21134)	21134..21896	-	763	Protein_31	plasmid SOS inhibition protein A	-
MW404_RS24535 (21893)	21893..22327	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
MW404_RS24540 (22382)	22382..24340	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B	-	1..67887	67887

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T242232 WP_001372321.1 NZ_CP095522:c19418-19293 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T242232 NZ_OW052189:3084177-3084551 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Nucleotide

• Length: 41 bp

>AT242232 NZ_CP095522:c19535-19495 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin