Detailed information of TA system TA242231

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4680277..4680879
• Replicon: chromosome
• Accession: NZ_CP095520
• Organism: Escherichia coli strain GN04171

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: MW404_RS23010
• Protein ID: WP_000897302.1
• Coordinates: 4680277..4680588 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MW404_RS23015
• Protein ID: WP_000356397.1
• Coordinates: 4680589..4680879 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW404_RS22985 (4676191)	4676191..4676790	+	600	WP_001296610.1	glucose-1-phosphatase	-
MW404_RS22990 (4676784)	4676784..4677656	+	873	WP_000920762.1	virulence factor BrkB family protein	-
MW404_RS22995 (4677653)	4677653..4678090	+	438	WP_000560981.1	D-aminoacyl-tRNA deacylase	-
MW404_RS23000 (4678135)	4678135..4679076	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
MW404_RS23005 (4679140)	4679140..4680048	-	909	WP_001385591.1	alpha/beta hydrolase	-
MW404_RS23010 (4680277)	4680277..4680588	+	312	WP_000897302.1	hypothetical protein	Toxin
MW404_RS23015 (4680589)	4680589..4680879	+	291	WP_000356397.1	NadS family protein	Antitoxin
MW404_RS23020 (4681238)	4681238..4681516	+	279	WP_001296612.1	hypothetical protein	-
MW404_RS23025 (4681913)	4681913..4682131	+	219	WP_001251293.1	CopG family transcriptional regulator	-
MW404_RS23030 (4682316)	4682316..4683056	-	741	WP_000608806.1	hypothetical protein	-
MW404_RS23035 (4683081)	4683081..4683929	-	849	WP_001038650.1	hypothetical protein	-
MW404_RS23040 (4684219)	4684219..4684461	+	243	WP_001068514.1	CopG family transcriptional regulator	-
MW404_RS23045 (4684643)	4684643..4685572	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T242231 WP_000897302.1 NZ_CP095520:4680277-4680588 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T242231 NZ_OW052189:c3075538-3075227 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT242231 WP_000356397.1 NZ_CP095520:4680589-4680879 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT242231 NZ_OW052189:c3075858-3075538 [Mycobacterium tuberculosis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Structures

Toxin

• AlphaFold DB: S1P416