Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3871787..3872586 Replicon chromosome
Accession NZ_CP095520
Organism Escherichia coli strain GN04171

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag MW404_RS19050 Protein ID WP_000347251.1
Coordinates 3872122..3872586 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag MW404_RS19045 Protein ID WP_001296435.1
Coordinates 3871787..3872122 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW404_RS19030 (3867572) 3867572..3868342 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
MW404_RS19035 (3868358) 3868358..3869692 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
MW404_RS19040 (3870067) 3870067..3871638 + 1572 WP_001273763.1 galactarate dehydratase -
MW404_RS19045 (3871787) 3871787..3872122 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MW404_RS19050 (3872122) 3872122..3872586 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MW404_RS19055 (3872641) 3872641..3873450 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MW404_RS19060 (3873699) 3873699..3874979 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
MW404_RS19065 (3875002) 3875002..3875475 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
MW404_RS19070 (3875486) 3875486..3876265 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
MW404_RS19075 (3876255) 3876255..3877133 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
MW404_RS19080 (3877151) 3877151..3877585 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T242229 WP_000347251.1 NZ_CP095520:3872122-3872586 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T242229 NZ_OW052189:2685900-2686328 [Mycobacterium tuberculosis]
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTCGCGGCTGACGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATC
GACGCATCTTCGAGATTCCGTCACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTT
CCGACGAACCCCGGTCCCAGACACCTCATGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGCGGCCATAGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCT
CGGTGCGCCACATTCGCCCGCCGCTCTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT242229 WP_001296435.1 NZ_CP095520:3871787-3872122 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT242229 NZ_OW052189:2685619-2685876 [Mycobacterium tuberculosis]
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAGGACGCCCTTCGGCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGAGCGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References