Detailed information of TA system TA242202

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 92565..92834
• Replicon: plasmid p2350-1
• Accession: NZ_CP095519
• Organism: Escherichia coli strain GN02350

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MW405_RS25455
• Protein ID: WP_001372321.1
• Coordinates: 92709..92834 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	92565..92630 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW405_RS25415	87589..88032	-	444	Protein_99	hypothetical protein	-
MW405_RS25420	88334..88861	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
MW405_RS25425	88919..89152	+	234	WP_000006003.1	DUF905 family protein	-
MW405_RS25430	89213..91177	+	1965	WP_001537566.1	ParB/RepB/Spo0J family partition protein	-
MW405_RS25435	91246..91680	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
MW405_RS25440	91677..92439	+	763	Protein_104	plasmid SOS inhibition protein A	-
-	92408..92632	+	225	NuclAT_0	-	-
-	92408..92632	+	225	NuclAT_0	-	-
-	92408..92632	+	225	NuclAT_0	-	-
-	92408..92632	+	225	NuclAT_0	-	-
MW405_RS25445	92417..92596	-	180	WP_001309233.1	hypothetical protein	-
-	92565..92630	-	66	-	-	Antitoxin
MW405_RS25450	92618..92767	+	150	Protein_106	plasmid maintenance protein Mok	-
MW405_RS25455	92709..92834	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
MW405_RS25460	93135..93431	-	297	Protein_108	hypothetical protein	-
MW405_RS25465	93731..94027	+	297	WP_001272251.1	hypothetical protein	-
MW405_RS25470	94137..94958	+	822	WP_001234445.1	DUF932 domain-containing protein	-
MW405_RS25475	95255..95857	-	603	Protein_111	transglycosylase SLT domain-containing protein	-
MW405_RS25480	96178..96561	+	384	WP_001151538.1	conjugal transfer relaxosome DNA-binding protein TraM	-
MW405_RS25485	96748..97437	+	690	WP_000283387.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / sul1 / blaSHV-48	senB	1..117950	117950

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T242202 WP_001372321.1 NZ_CP095519:92709-92834 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T242202 NZ_OW052189:c534733-534377 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT242202 NZ_CP095519:c92630-92565 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin