Detailed information of TA system TA242198

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 16872..17125
• Replicon: plasmid p2350-1
• Accession: NZ_CP095519
• Organism: Escherichia coli strain GN02350

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: MW405_RS24985
• Protein ID: WP_001336447.1
• Coordinates: 16976..17125 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	16872..16928 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW405_RS24955 (12774)	12774..13520	+	747	WP_000205709.1	conjugal transfer pilus acetylase TraX	-
MW405_RS24960 (13575)	13575..14132	+	558	WP_000139329.1	fertility inhibition protein FinO	-
MW405_RS24965 (14284)	14284..14487	+	204	WP_001336517.1	hypothetical protein	-
MW405_RS24970 (15240)	15240..15701	+	462	WP_000760080.1	thermonuclease family protein	-
MW405_RS24975 (15804)	15804..16187	+	384	WP_001109264.1	hypothetical protein	-
MW405_RS24980 (16340)	16340..16777	+	438	WP_000872609.1	hypothetical protein	-
- (16872)	16872..16928	-	57	NuclAT_1	-	Antitoxin
- (16872)	16872..16928	-	57	NuclAT_1	-	Antitoxin
- (16872)	16872..16928	-	57	NuclAT_1	-	Antitoxin
- (16872)	16872..16928	-	57	NuclAT_1	-	Antitoxin
MW405_RS24985 (16976)	16976..17125	+	150	WP_001336447.1	Hok/Gef family protein	Toxin
MW405_RS24990 (17410)	17410..17667	+	258	WP_000084404.1	replication regulatory protein RepA	-
MW405_RS24995 (17901)	17901..17975	+	75	WP_001365705.1	RepA leader peptide Tap	-
MW405_RS25000 (17968)	17968..18825	+	858	WP_001537577.1	incFII family plasmid replication initiator RepA	-
MW405_RS25005 (19526)	19526..19666	+	141	WP_001333237.1	hypothetical protein	-
MW405_RS25010 (19764)	19764..20417	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
MW405_RS25015 (20510)	20510..20767	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
MW405_RS25020 (20700)	20700..21101	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / sul1 / blaSHV-48	senB	1..117950	117950

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T242198 WP_001336447.1 NZ_CP095519:16976-17125 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T242198 NZ_OW052189:355670-356110 [Mycobacterium tuberculosis]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA

Antitoxin

Nucleotide

• Length: 57 bp

>AT242198 NZ_CP095519:c16928-16872 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin