Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4880019..4880581 Replicon chromosome
Accession NZ_CP095518
Organism Escherichia coli strain GN02350

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag MW405_RS23185 Protein ID WP_000605675.1
Coordinates 4880019..4880297 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag MW405_RS23190 Protein ID WP_000781370.1
Coordinates 4880297..4880581 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW405_RS23160 (4875607) 4875607..4876038 - 432 WP_000152305.1 peroxiredoxin OsmC -
MW405_RS23165 (4876383) 4876383..4876598 + 216 WP_000495766.1 biofilm-dependent modulation protein -
MW405_RS23170 (4876700) 4876700..4876837 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
MW405_RS23175 (4876993) 4876993..4878690 + 1698 WP_000433462.1 malate dehydrogenase -
MW405_RS23180 (4878823) 4878823..4879833 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
MW405_RS23185 (4880019) 4880019..4880297 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MW405_RS23190 (4880297) 4880297..4880581 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
MW405_RS23195 (4880810) 4880810..4881463 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
MW405_RS23200 (4881456) 4881456..4882340 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
MW405_RS23205 (4882353) 4882353..4885400 - 3048 WP_077634457.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T242197 WP_000605675.1 NZ_CP095518:4880019-4880297 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T242197 NZ_OW052189:c295449-295054 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT242197 WP_000781370.1 NZ_CP095518:4880297-4880581 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT242197 NZ_OW052189:c295691-295446 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References