Detailed information of TA system TA242185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3506282..3507009
• Replicon: chromosome
• Accession: NZ_CP095518
• Organism: Escherichia coli strain GN02350

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: MW405_RS16670
• Protein ID: WP_000547563.1
• Coordinates: 3506282..3506593 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MW405_RS16675
• Protein ID: WP_000126304.1
• Coordinates: 3506590..3507009 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW405_RS16640 (3501426)	3501426..3503135	+	1710	WP_001288123.1	formate hydrogenlyase subunit HycE	-
MW405_RS16645 (3503145)	3503145..3503687	+	543	WP_000493800.1	formate hydrogenlyase subunit HycF	-
MW405_RS16650 (3503687)	3503687..3504454	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
MW405_RS16655 (3504451)	3504451..3504861	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
MW405_RS16660 (3504854)	3504854..3505321	+	468	WP_000132955.1	hydrogenase maturation peptidase HycI	-
MW405_RS16665 (3505364)	3505364..3506119	+	756	WP_001314074.1	hypothetical protein	-
MW405_RS16670 (3506282)	3506282..3506593	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
MW405_RS16675 (3506590)	3506590..3507009	+	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
MW405_RS16680 (3507101)	3507101..3507529	-	429	WP_000536066.1	DUF4259 domain-containing protein	-
MW405_RS16685 (3507914)	3507914..3508441	+	528	WP_001078777.1	electron transport protein HydN	-
MW405_RS16690 (3508594)	3508594..3510846	+	2253	WP_001467844.1	carbamoyltransferase HypF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3477436..3516354	38918

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T242185 WP_000547563.1 NZ_CP095518:3506282-3506593 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T242185 NZ_OW052188:c3689253-3688876 [Mycobacterium tuberculosis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT242185 WP_000126304.1 NZ_CP095518:3506590-3507009 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT242185 NZ_OW052188:c3688834-3688385 [Mycobacterium tuberculosis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22