Detailed information of TA system TA242165

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41536..41789
• Replicon: plasmid p2323-2
• Accession: NZ_CP095517
• Organism: Escherichia coli strain GN02323

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: MW403_RS25130
• Protein ID: WP_001336447.1
• Coordinates: 41640..41789 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	41536..41592 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW403_RS25100 (37448)	37448..38194	+	747	WP_019842127.1	conjugal transfer pilus acetylase TraX	-
MW403_RS25105 (38249)	38249..38806	+	558	WP_000139329.1	fertility inhibition protein FinO	-
MW403_RS25110 (38958)	38958..39161	+	204	WP_001336517.1	hypothetical protein	-
MW403_RS25115 (39903)	39903..40364	+	462	WP_019842128.1	thermonuclease family protein	-
MW403_RS25120 (40633)	40633..40851	+	219	WP_023150739.1	hypothetical protein	-
MW403_RS25125 (41004)	41004..41441	+	438	WP_000872609.1	hypothetical protein	-
- (41536)	41536..41592	-	57	NuclAT_1	-	Antitoxin
- (41536)	41536..41592	-	57	NuclAT_1	-	Antitoxin
- (41536)	41536..41592	-	57	NuclAT_1	-	Antitoxin
- (41536)	41536..41592	-	57	NuclAT_1	-	Antitoxin
MW403_RS25130 (41640)	41640..41789	+	150	WP_001336447.1	Hok/Gef family protein	Toxin
MW403_RS25135 (42074)	42074..42331	+	258	WP_000084404.1	replication regulatory protein RepA	-
MW403_RS25140 (42567)	42567..42641	+	75	WP_001365705.1	RepA leader peptide Tap	-
MW403_RS25145 (42634)	42634..43491	+	858	WP_000130646.1	incFII family plasmid replication initiator RepA	-
MW403_RS25150 (44404)	44404..44541	+	138	Protein_54	XRE family transcriptional regulator	-
MW403_RS25155 (44561)	44561..44824	+	264	WP_001089473.1	hypothetical protein	-
MW403_RS25160 (44814)	44814..45113	+	300	WP_001528606.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MW403_RS25165 (45149)	45149..45814	-	666	WP_001535733.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..74109	74109

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T242165 WP_001336447.1 NZ_CP095517:41640-41789 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T242165 NZ_OW052188:c2715091-2714828 [Mycobacterium tuberculosis]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin

Nucleotide

• Length: 57 bp

>AT242165 NZ_CP095517:c41592-41536 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin