Detailed information of TA system TA242162

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1255..1524
• Replicon: plasmid p2323-2
• Accession: NZ_CP095517
• Organism: Escherichia coli strain GN02323

Toxin (Protein)

• Gene name: hok
• Uniprot ID: G9G195
• Locus tag: MW403_RS24895
• Protein ID: WP_001323520.1
• Coordinates: 1408..1524 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	1255..1320 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW403_RS24880	1..370	+	370	WP_032327614.1	conjugation system SOS inhibitor PsiB	-
MW403_RS24885	367..1129	+	763	Protein_1	plasmid SOS inhibition protein A	-
-	1098..1322	+	225	NuclAT_0	-	-
-	1098..1322	+	225	NuclAT_0	-	-
-	1098..1322	+	225	NuclAT_0	-	-
-	1098..1322	+	225	NuclAT_0	-	-
-	1255..1320	-	66	-	-	Antitoxin
MW403_RS24890	1308..1457	+	150	Protein_2	plasmid maintenance protein Mok	-
MW403_RS24895	1408..1524	+	117	WP_001323520.1	type I toxin-antitoxin system Hok family toxin	Toxin
MW403_RS24900	1843..2140	-	298	Protein_4	hypothetical protein	-
MW403_RS24905	2440..2736	+	297	WP_001272251.1	hypothetical protein	-
MW403_RS24910	2847..3668	+	822	WP_001234445.1	DUF932 domain-containing protein	-
MW403_RS24915	3965..4567	-	603	Protein_7	transglycosylase SLT domain-containing protein	-
MW403_RS24920	4888..5271	+	384	WP_001151538.1	conjugal transfer relaxosome DNA-binding protein TraM	-
MW403_RS24925	5458..6147	+	690	WP_016239105.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..74109	74109

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4455.23 Da
• Isoelectric Point: 8.5110

>T242162 WP_001323520.1 NZ_CP095517:1408-1524 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T242162 NZ_OW052188:2185520-2185948 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT242162 NZ_CP095517:c1320-1255 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: G9G195

Antitoxin