Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3769078..3769805 Replicon chromosome
Accession NZ_CP095514
Organism Escherichia coli strain GN02323

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag MW403_RS17995 Protein ID WP_000547555.1
Coordinates 3769494..3769805 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag MW403_RS17990 Protein ID WP_000126293.1
Coordinates 3769078..3769497 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW403_RS17975 (3765241) 3765241..3767493 - 2253 WP_001107659.1 carbamoyltransferase HypF -
MW403_RS17980 (3767646) 3767646..3768173 - 528 WP_001078777.1 electron transport protein HydN -
MW403_RS17985 (3768558) 3768558..3768986 + 429 WP_000536064.1 hypothetical protein -
MW403_RS17990 (3769078) 3769078..3769497 - 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
MW403_RS17995 (3769494) 3769494..3769805 - 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
MW403_RS18000 (3769968) 3769968..3770723 - 756 WP_001305268.1 hypothetical protein -
MW403_RS18005 (3770766) 3770766..3771236 - 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
MW403_RS18010 (3771229) 3771229..3771639 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
MW403_RS18015 (3771636) 3771636..3772403 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
MW403_RS18020 (3772403) 3772403..3772945 - 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
MW403_RS18025 (3772955) 3772955..3774664 - 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T242156 WP_000547555.1 NZ_CP095514:c3769805-3769494 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T242156 NZ_OW052188:c1113293-1112991 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT242156 WP_000126293.1 NZ_CP095514:c3769497-3769078 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT242156 NZ_OW052188:c1112943-1112722 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References