Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2400758..2401320 Replicon chromosome
Accession NZ_CP095514
Organism Escherichia coli strain GN02323

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag MW403_RS11550 Protein ID WP_000605675.1
Coordinates 2401042..2401320 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag MW403_RS11545 Protein ID WP_000781370.1
Coordinates 2400758..2401042 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW403_RS11530 (2395939) 2395939..2398986 + 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -
MW403_RS11535 (2398999) 2398999..2399883 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
MW403_RS11540 (2399876) 2399876..2400529 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
MW403_RS11545 (2400758) 2400758..2401042 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
MW403_RS11550 (2401042) 2401042..2401320 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MW403_RS11555 (2401506) 2401506..2402516 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
MW403_RS11560 (2402650) 2402650..2404347 - 1698 WP_000433460.1 malate dehydrogenase -
MW403_RS11565 (2404504) 2404504..2404641 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
MW403_RS11570 (2404743) 2404743..2404958 - 216 WP_000495766.1 biofilm-dependent modulation protein -
MW403_RS11575 (2405303) 2405303..2405734 + 432 WP_000152307.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T242144 WP_000605675.1 NZ_CP095514:c2401320-2401042 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T242144 NZ_OW052188:720701-721009 [Mycobacterium tuberculosis]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT242144 WP_000781370.1 NZ_CP095514:c2401042-2400758 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT242144 NZ_OW052188:720469-720714 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References