Detailed information of TA system TA242133

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 127679..127948
• Replicon: plasmid p2270-1
• Accession: NZ_CP095511
• Organism: Escherichia coli strain GN02270

Toxin (Protein)

• Gene name: hok
• Uniprot ID: G9G195
• Locus tag: MW401_RS25895
• Protein ID: WP_001323520.1
• Coordinates: 127832..127948 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	127679..127744 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW401_RS25865	124532..124837	+	306	Protein_155	conjugation system SOS inhibitor PsiB	-
MW401_RS25870	125026..126213	-	1188	WP_001554936.1	IS91 family transposase	-
MW401_RS25875	126213..126578	-	366	WP_000091308.1	hypothetical protein	-
MW401_RS25880	126660..126794	+	135	Protein_158	conjugation system SOS inhibitor PsiB	-
MW401_RS25885	126791..127553	+	763	Protein_159	plasmid SOS inhibition protein A	-
-	127522..127746	+	225	NuclAT_0	-	-
-	127522..127746	+	225	NuclAT_0	-	-
-	127522..127746	+	225	NuclAT_0	-	-
-	127522..127746	+	225	NuclAT_0	-	-
-	127679..127744	-	66	-	-	Antitoxin
MW401_RS25890	127732..127881	+	150	Protein_160	plasmid maintenance protein Mok	-
MW401_RS25895	127832..127948	+	117	WP_001323520.1	type I toxin-antitoxin system Hok family toxin	Toxin
MW401_RS25900	128267..128564	-	298	Protein_162	hypothetical protein	-
MW401_RS25905	128864..129160	+	297	WP_001272251.1	hypothetical protein	-
MW401_RS25910	129271..130092	+	822	WP_001234445.1	DUF932 domain-containing protein	-
MW401_RS25915	130389..130991	-	603	Protein_165	transglycosylase SLT domain-containing protein	-
MW401_RS25920	131312..131695	+	384	WP_001151538.1	conjugal transfer relaxosome DNA-binding protein TraM	-
MW401_RS25925	131882..132571	+	690	WP_016239105.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..164623	164623
	-	flank	IS/Tn	-	-	125026..126213	1187

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4455.23 Da
• Isoelectric Point: 8.5110

>T242133 WP_001323520.1 NZ_CP095511:127832-127948 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T242133 NZ_OW011623:3572594-3572971 [Thauera sp. PIV-1]
ATGGTCGAAAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCTACAAGGATCTGATGACGTTGCCGCCCGATGTGCGCCG
GCGTTTTGGTTACGCGCTGTCGCTGGCACAGATGGGCGACCAGGACGATGCGGCAAAGGTGCTCAAGGGCTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACGTATCGAGCGGTCTACACGGTCAAGTTCGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGGATCGCCACGCCGAAGGCAGACATGGACATCATCCGCGCTCG
GCTGAAAGTGGCCGAGGCATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT242133 NZ_CP095511:c127744-127679 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: G9G195

Antitoxin