Detailed information of TA system TA242130

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53722..54144
• Replicon: plasmid p2270-1
• Accession: NZ_CP095511
• Organism: Escherichia coli strain GN02270

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MW401_RS25430
• Protein ID: WP_096937776.1
• Coordinates: 54019..54144 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	53722..53921 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW401_RS25395 (48917)	48917..49369	-	453	WP_024185774.1	hypothetical protein	-
MW401_RS25400 (49671)	49671..50198	+	528	WP_000290833.1	single-stranded DNA-binding protein	-
MW401_RS25405 (50255)	50255..50488	+	234	WP_000006018.1	DUF905 domain-containing protein	-
MW401_RS25410 (50547)	50547..52505	+	1959	WP_001145501.1	ParB/RepB/Spo0J family partition protein	-
MW401_RS25415 (52560)	52560..52994	+	435	WP_000845910.1	conjugation system SOS inhibitor PsiB	-
MW401_RS25420 (52991)	52991..53754	+	764	Protein_66	plasmid SOS inhibition protein A	-
- (53722)	53722..53921	+	200	NuclAT_1	-	Antitoxin
- (53722)	53722..53921	+	200	NuclAT_1	-	Antitoxin
- (53722)	53722..53921	+	200	NuclAT_1	-	Antitoxin
- (53722)	53722..53921	+	200	NuclAT_1	-	Antitoxin
MW401_RS25425 (53928)	53928..54077	+	150	Protein_67	DUF5431 family protein	-
MW401_RS25430 (54019)	54019..54144	+	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
MW401_RS25435 (54462)	54462..54759	-	298	Protein_69	hypothetical protein	-
MW401_RS25440 (55060)	55060..55347	+	288	WP_000107526.1	hypothetical protein	-
MW401_RS25445 (55466)	55466..56287	+	822	WP_001234489.1	DUF932 domain-containing protein	-
MW401_RS25450 (56583)	56583..57185	-	603	WP_001323326.1	transglycosylase SLT domain-containing protein	-
MW401_RS25455 (57516)	57516..57899	+	384	WP_001151562.1	conjugal transfer relaxosome DNA-binding protein TraM	-
MW401_RS25460 (58093)	58093..58779	+	687	WP_000332487.1	PAS domain-containing protein	-
MW401_RS25465 (58873)	58873..59100	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..164623	164623

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T242130 WP_096937776.1 NZ_CP095511:54019-54144 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T242130 NZ_OW011623:c1853355-1853053 [Thauera sp. PIV-1]
ATGAGCTATAAGCTGCGCTTTCACAGCCTTGCGCTCGCGGAATGGAGAAAGCTCGACGGTAGCCTGCGGCTGCAGTTCAA
GAAGAAGCTCGAAGAGCGCCTGATCACCCCTCGCGTCCCCTCGTCTGCCCTCTCCGGCATGCCAGACTGCTACAAGATCA
AGCTGCGCGCAGTCGGCTACCGCCTGGTCTATCGCGTCGATGAAGACGTGGTGTTCGTGACCGTCATCTCGGTCGGCCTG
CGAGACAAGCAGCGAGTCTATTCAAAGGCCCACGCCCGCCTGGATGAGCCAGAGCAGGACTGA

Antitoxin

Nucleotide

• Length: 200 bp

>AT242130 NZ_CP095511:53722-53921 [Escherichia coli]
TCACAACGGATTGCCCGTAAACAGCCTGAATGAGCGAGTTATTTTCAGGAAAAGTGAATGTGGTCAGCGTGCTGGTATAT
GGGCTATGATGTGCCCGGCGCTTGAGGTTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGG
ACAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin