Detailed information of TA system TA242123

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 4653271..4653683
• Replicon: chromosome
• Accession: NZ_CP095510
• Organism: Escherichia coli strain GN02270

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: MW401_RS22540
• Protein ID: WP_000132601.1
• Coordinates: 4653342..4653683 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	4653271..4653347 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW401_RS22530 (4650134)	4650134..4651723	+	1590	WP_001063200.1	type I restriction-modification system methyltransferase	-
MW401_RS22535 (4651720)	4651720..4653114	+	1395	WP_001272445.1	type I restriction-modification system specificity subunit	-
- (4653271)	4653271..4653347	-	77	NuclAT_12	-	Antitoxin
- (4653271)	4653271..4653347	-	77	NuclAT_12	-	Antitoxin
- (4653271)	4653271..4653347	-	77	NuclAT_12	-	Antitoxin
- (4653271)	4653271..4653347	-	77	NuclAT_12	-	Antitoxin
- (4653271)	4653271..4653347	-	77	NuclAT_13	-	Antitoxin
- (4653271)	4653271..4653347	-	77	NuclAT_13	-	Antitoxin
- (4653271)	4653271..4653347	-	77	NuclAT_13	-	Antitoxin
- (4653271)	4653271..4653347	-	77	NuclAT_13	-	Antitoxin
MW401_RS22540 (4653342)	4653342..4653683	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
MW401_RS22545 (4653845)	4653845..4655224	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
MW401_RS22550 (4655224)	4655224..4656270	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T242123 WP_000132601.1 NZ_CP095510:4653342-4653683 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T242123 NZ_OV996158:125389-125658 [Enterococcus mundtii]
TTGGGATACCTTATAGAAATTAAAAAAAATGCAAAAAAAGATTTTACTAAACTTAAAAGTGCAGGGTTACATAAAAAGTT
TGATGAATTACTCAAGGTCATTCGAAAGAATCCTTTTCAGTCACCGCCACCGTATGAAAAACTAGTAGGAATTCCGTATT
ACTCAAGAAGAATCAACATTCAGCATAGACTAGTTTATAGTGTGGACATTGAGAGGGAAATAGTAACAATTCTTTCCGTC
TGGTCTCATTATGAGCGTGGGTTGTTTTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT242123 NZ_CP095510:c4653347-4653271 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin