Detailed information of TA system TA242120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4263172..4263822
• Replicon: chromosome
• Accession: NZ_CP095510
• Organism: Escherichia coli strain GN02270

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MW401_RS20720
• Protein ID: WP_000263532.1
• Coordinates: 4263481..4263822 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U9YXQ7
• Locus tag: MW401_RS20715
• Protein ID: WP_000212552.1
• Coordinates: 4263172..4263471 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW401_RS20690 (4258620)	4258620..4258931	-	312	WP_000415004.1	hypothetical protein	-
MW401_RS20695 (4258936)	4258936..4259328	-	393	WP_000285322.1	flagellar export chaperone FliS	-
MW401_RS20700 (4259351)	4259351..4260667	-	1317	WP_000609678.1	flagellar filament capping protein FliD	-
MW401_RS20705 (4260872)	4260872..4261786	-	915	WP_000949083.1	lateral flagellin LafA	-
MW401_RS20710 (4262272)	4262272..4263114	+	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
MW401_RS20715 (4263172)	4263172..4263471	-	300	WP_000212552.1	helix-turn-helix transcriptional regulator	Antitoxin
MW401_RS20720 (4263481)	4263481..4263822	-	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MW401_RS20725 (4263898)	4263898..4264875	-	978	WP_001195938.1	hypothetical protein	-
MW401_RS20730 (4264892)	4264892..4265821	-	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
MW401_RS20735 (4265836)	4265836..4267212	-	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
MW401_RS20740 (4267388)	4267388..4267687	-	300	WP_000867281.1	rod-binding protein	-
MW401_RS20745 (4267687)	4267687..4268787	-	1101	WP_001181874.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T242120 WP_000263532.1 NZ_CP095510:c4263822-4263481 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T242120 NZ_OV754011:48189-48671 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11004.86 Da
• Isoelectric Point: 9.7665

>AT242120 WP_000212552.1 NZ_CP095510:c4263471-4263172 [Escherichia coli]
MATLKELMAKQSPESQVRIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT242120 NZ_OV754011:47935-48201 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: U9YXQ7