24212

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) symE-agrB/SymE(toxin)
Location 5466976..5467388 Replicon chromosome
Accession NC_013008
Organism Escherichia coli O157:H7 str. TW14359

Toxin (Protein)


Gene name symE Uniprot ID Q8FA88
Locus tag ECSP_RS28230 Protein ID WP_000132630.1
Coordinates 5466976..5467317 (-) Length 114 a.a.

Antitoxin (RNA)


Gene name agrB
Locus tag -
Coordinates 5467312..5467388 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECSP_RS33835 5462544..5462744 + 201 WP_001310455.1 hypothetical protein -
ECSP_RS28210 5462987..5464399 - 1413 WP_000199295.1 PLP-dependent aminotransferase family protein -
ECSP_RS28215 5464576..5464740 + 165 WP_000394274.1 DUF1127 domain-containing protein -
ECSP_RS28220 5464837..5465718 + 882 WP_001304007.1 DUF262 domain-containing protein -
ECSP_RS31595 5465766..5466929 + 1164 WP_001304006.1 DUF1524 domain-containing protein -
ECSP_RS28230 5466976..5467317 - 342 WP_000132630.1 endoribonuclease SymE Toxin
- 5467312..5467388 + 77 NuclAT_14 - Antitoxin
- 5467312..5467388 + 77 NuclAT_14 - Antitoxin
- 5467312..5467388 + 77 NuclAT_14 - Antitoxin
- 5467312..5467388 + 77 NuclAT_14 - Antitoxin
- 5467312..5467388 + 77 NuclAT_15 - Antitoxin
- 5467312..5467388 + 77 NuclAT_15 - Antitoxin
- 5467312..5467388 + 77 NuclAT_15 - Antitoxin
- 5467312..5467388 + 77 NuclAT_15 - Antitoxin
ECSP_RS28235 5467538..5469292 - 1755 WP_000110082.1 restriction endonuclease subunit S -
ECSP_RS28240 5469292..5470761 - 1470 WP_001302468.1 type I restriction-modification system subunit M -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 5456469..5475189 18720


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SymE-like

(20-71)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12294.13 Da        Isoelectric Point: 8.4982

>T24212 WP_000132630.1 NC_013008:c5467317-5466976 [Escherichia coli O157:H7 str. TW14359]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Download         Length: 342 bp

>T24212 NC_013008:c5467317-5466976 [Escherichia coli O157:H7 str. TW14359]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin


Download         Length: 77 bp

>AT24212 NC_013008:5467312-5467388 [Escherichia coli O157:H7 str. TW14359]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y5T0

Antitoxin

Download structure file

References