Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2862106..2862668 Replicon chromosome
Accession NZ_CP095510
Organism Escherichia coli strain GN02270

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag MW401_RS13705 Protein ID WP_000605676.1
Coordinates 2862106..2862384 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag MW401_RS13710 Protein ID WP_000781370.1
Coordinates 2862384..2862668 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW401_RS13680 (2857692) 2857692..2858123 - 432 WP_000152305.1 peroxiredoxin OsmC -
MW401_RS13685 (2858468) 2858468..2858683 + 216 WP_000495766.1 biofilm-dependent modulation protein -
MW401_RS13690 (2858785) 2858785..2858922 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
MW401_RS13695 (2859079) 2859079..2860776 + 1698 WP_000433464.1 malate dehydrogenase -
MW401_RS13700 (2860910) 2860910..2861920 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
MW401_RS13705 (2862106) 2862106..2862384 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MW401_RS13710 (2862384) 2862384..2862668 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
MW401_RS13715 (2862896) 2862896..2863549 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
MW401_RS13720 (2863542) 2863542..2864426 - 885 WP_001240565.1 formate dehydrogenase N subunit beta -
MW401_RS13725 (2864439) 2864439..2867486 - 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T242115 WP_000605676.1 NZ_CP095510:2862106-2862384 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T242115 NZ_OV754010:c4617469-4616936 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTTTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT242115 WP_000781370.1 NZ_CP095510:2862384-2862668 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT242115 NZ_OV754010:c4617746-4617480 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References