Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 923856..924583 Replicon chromosome
Accession NZ_CP095510
Organism Escherichia coli strain GN02270

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag MW401_RS04510 Protein ID WP_000550189.1
Coordinates 923856..924170 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID D3GWQ0
Locus tag MW401_RS04515 Protein ID WP_000560253.1
Coordinates 924167..924583 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW401_RS04490 (920022) 920022..921008 - 987 WP_001377074.1 Gfo/Idh/MocA family oxidoreductase -
MW401_RS04495 (921087) 921087..921770 - 684 WP_001183054.1 vancomycin high temperature exclusion protein -
MW401_RS04500 (921847) 921847..922350 - 504 WP_001309773.1 M48 family metallopeptidase -
MW401_RS04505 (922435) 922435..923571 + 1137 WP_000018677.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
MW401_RS04510 (923856) 923856..924170 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
MW401_RS04515 (924167) 924167..924583 + 417 WP_000560253.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
MW401_RS04520 (924628) 924628..926646 - 2019 WP_000121438.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
MW401_RS04525 (926817) 926817..929168 - 2352 WP_000695439.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T242100 WP_000550189.1 NZ_CP095510:923856-924170 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T242100 NZ_OV753987:1945751-1945853 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14965.39 Da        Isoelectric Point: 4.4547

>AT242100 WP_000560253.1 NZ_CP095510:924167-924583 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT242100 NZ_OV753987:c1945860-1945715 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure
AlphaFold DB A0A829IXD3

References