Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 923856..924583 | Replicon | chromosome |
| Accession | NZ_CP095510 | ||
| Organism | Escherichia coli strain GN02270 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U9ZMR4 |
| Locus tag | MW401_RS04510 | Protein ID | WP_000550189.1 |
| Coordinates | 923856..924170 (+) | Length | 105 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | D3GWQ0 |
| Locus tag | MW401_RS04515 | Protein ID | WP_000560253.1 |
| Coordinates | 924167..924583 (+) | Length | 139 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MW401_RS04490 (920022) | 920022..921008 | - | 987 | WP_001377074.1 | Gfo/Idh/MocA family oxidoreductase | - |
| MW401_RS04495 (921087) | 921087..921770 | - | 684 | WP_001183054.1 | vancomycin high temperature exclusion protein | - |
| MW401_RS04500 (921847) | 921847..922350 | - | 504 | WP_001309773.1 | M48 family metallopeptidase | - |
| MW401_RS04505 (922435) | 922435..923571 | + | 1137 | WP_000018677.1 | 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG | - |
| MW401_RS04510 (923856) | 923856..924170 | + | 315 | WP_000550189.1 | type II toxin-antitoxin system toxin HigB | Toxin |
| MW401_RS04515 (924167) | 924167..924583 | + | 417 | WP_000560253.1 | type II toxin-antitoxin system antitoxin HigA | Antitoxin |
| MW401_RS04520 (924628) | 924628..926646 | - | 2019 | WP_000121438.1 | NADPH-dependent 2,4-dienoyl-CoA reductase | - |
| MW401_RS04525 (926817) | 926817..929168 | - | 2352 | WP_000695439.1 | alpha-glucosidase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 105 a.a. Molecular weight: 12103.10 Da Isoelectric Point: 10.0409
>T242100 WP_000550189.1 NZ_CP095510:923856-924170 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK
Download Length: 315 bp
>T242100 NZ_OV753987:1945751-1945853 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT
Antitoxin
Download Length: 139 a.a. Molecular weight: 14965.39 Da Isoelectric Point: 4.4547
>AT242100 WP_000560253.1 NZ_CP095510:924167-924583 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID
MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID
Download Length: 417 bp
>AT242100 NZ_OV753987:c1945860-1945715 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| PDB | 6KMQ | |
| PDB | 5IFG | |
| PDB | 5YCL | |
| PDB | 6KML |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829IXD3 |