24210

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5238295..5238792 Replicon chromosome
Accession NC_013008
Organism Escherichia coli O157:H7 str. TW14359

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECSP_RS27170 Protein ID WP_001173343.1
Coordinates 5238619..5238792 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ECSP_RS27165 Protein ID WP_000398619.1
Coordinates 5238295..5238591 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECSP_RS27140 5235026..5235745 - 720 WP_000611288.1 two-component system response regulator DcuR -
ECSP_RS27145 5235742..5237373 - 1632 WP_001216477.1 sensor histidine kinase -
ECSP_RS27150 5237554..5237784 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
ECSP_RS27155 5237796..5238068 + 273 WP_000405647.1 N-acetyltransferase -
ECSP_RS27165 5238295..5238591 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECSP_RS27170 5238619..5238792 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECSP_RS27175 5238911..5240428 - 1518 WP_001295074.1 lysine--tRNA ligase -
ECSP_RS27180 5240665..5242122 - 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 5238295..5247646 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T24210 WP_001173343.1 NC_013008:5238619-5238792 [Escherichia coli O157:H7 str. TW14359]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T24210 NC_013008:5238619-5238792 [Escherichia coli O157:H7 str. TW14359]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT24210 WP_000398619.1 NC_013008:5238295-5238591 [Escherichia coli O157:H7 str. TW14359]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT24210 NC_013008:5238295-5238591 [Escherichia coli O157:H7 str. TW14359]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References