Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4102396..4103075 Replicon chromosome
Accession NZ_CP095505
Organism Escherichia coli strain GN02050

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag MW398_RS20945 Protein ID WP_000057524.1
Coordinates 4102396..4102698 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag MW398_RS20950 Protein ID WP_000806442.1
Coordinates 4102734..4103075 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW398_RS20920 (4097772) 4097772..4099424 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
MW398_RS20925 (4099462) 4099462..4099965 - 504 WP_000667000.1 hypothetical protein -
MW398_RS20930 (4099962) 4099962..4100762 - 801 WP_000439798.1 hypothetical protein -
MW398_RS20935 (4100786) 4100786..4101265 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
MW398_RS20940 (4101469) 4101469..4102263 - 795 WP_000365177.1 TraB/GumN family protein -
MW398_RS20945 (4102396) 4102396..4102698 + 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MW398_RS20950 (4102734) 4102734..4103075 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
MW398_RS20955 (4103133) 4103133..4105637 - 2505 WP_021513535.1 copper-exporting P-type ATPase CopA -
MW398_RS20960 (4105899) 4105899..4106831 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T242089 WP_000057524.1 NZ_CP095505:4102396-4102698 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T242089 NZ_OV753634:1998794-1998896 [Klebsiella quasipneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT242089 WP_000806442.1 NZ_CP095505:4102734-4103075 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT242089 NZ_OV753634:c1998900-1998753 [Klebsiella quasipneumoniae]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGTCTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References