Detailed information of TA system TA242087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3871472..3872166
• Replicon: chromosome
• Accession: NZ_CP095505
• Organism: Escherichia coli strain GN02050

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: MW398_RS19880
• Protein ID: WP_001263500.1
• Coordinates: 3871768..3872166 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: MW398_RS19875
• Protein ID: WP_000554758.1
• Coordinates: 3871472..3871765 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW398_RS19850 (3866910)	3866910..3867407	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
MW398_RS19855 (3867402)	3867402..3867746	-	345	WP_125093574.1	hypothetical protein	-
MW398_RS19860 (3867825)	3867825..3869537	-	1713	Protein_3696	flagellar biosynthesis protein FlhA	-
MW398_RS19865 (3869509)	3869509..3870294	+	786	WP_061301231.1	putative lateral flagellar export/assembly protein LafU	-
MW398_RS19870 (3870365)	3870365..3871420	+	1056	WP_001226168.1	DNA polymerase IV	-
MW398_RS19875 (3871472)	3871472..3871765	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
MW398_RS19880 (3871768)	3871768..3872166	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
MW398_RS19885 (3872176)	3872176..3872628	+	453	WP_071778924.1	GNAT family N-acetyltransferase	-
MW398_RS19890 (3872818)	3872818..3873957	+	1140	WP_000521561.1	RNA ligase RtcB family protein	-
MW398_RS19895 (3873954)	3873954..3874568	+	615	WP_000602123.1	peptide chain release factor H	-
MW398_RS19900 (3874625)	3874625..3876082	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
MW398_RS19905 (3876343)	3876343..3876801	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3850587..3872628	22041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T242087 WP_001263500.1 NZ_CP095505:3871768-3872166 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T242087 NZ_OV648025:c2358494-2358243 [Staphylococcus argenteus]
ATGATTGTAAAAAACTATTCTTACGCTAGACAGAATTTAAAGACGCTTATGACAAAGGTAAATGACGATAGTGATGTGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATTATGTCAGAAGCAGATTATAATTCTATAATTGAAACCCTTTACC
TTCAACAGAATCCAAATAATGCTGAACACTTAGCCCAATCAATTGCTGACTTAGAACGAGGCAAAATGGTAACGAAAGAA
ATAGATGTGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT242087 WP_000554758.1 NZ_CP095505:3871472-3871765 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT242087 NZ_OV648025:c2358243-2357977 [Staphylococcus argenteus]
ATGGCTAGGTTAAACATTACGTTCTCGCCTCAGGCATTTGAAGATTATGAGTATTTTCAGCAGAACGATAAAAAAATGGT
AAAGAAGATTAATGAGTTACTTAAAAGCATTGATAGAAATGGTGCATTGCAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATTTATTTGGCTATTATAGTAGACGTATCAATCACGAACATAGGTTGGTTTATACAGTTGACGATAATCACATAAAA
ATAGCATCCTGTAGATACCATTATTAG

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3