Detailed information of TA system TA242080

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2845234..2845836
• Replicon: chromosome
• Accession: NZ_CP095505
• Organism: Escherichia coli strain GN02050

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: MW398_RS14970
• Protein ID: WP_000897302.1
• Coordinates: 2845234..2845545 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MW398_RS14975
• Protein ID: WP_000356397.1
• Coordinates: 2845546..2845836 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW398_RS14940 (2840264)	2840264..2841049	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
MW398_RS14945 (2841148)	2841148..2841747	+	600	WP_001314324.1	glucose-1-phosphatase	-
MW398_RS14950 (2841741)	2841741..2842613	+	873	WP_000920762.1	virulence factor BrkB family protein	-
MW398_RS14955 (2842610)	2842610..2843047	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
MW398_RS14960 (2843092)	2843092..2844033	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
MW398_RS14965 (2844097)	2844097..2845005	-	909	WP_001331553.1	alpha/beta hydrolase	-
MW398_RS14970 (2845234)	2845234..2845545	+	312	WP_000897302.1	hypothetical protein	Toxin
MW398_RS14975 (2845546)	2845546..2845836	+	291	WP_000356397.1	NadS family protein	Antitoxin
MW398_RS14980 (2846195)	2846195..2846473	+	279	WP_001296612.1	hypothetical protein	-
MW398_RS14985 (2846869)	2846869..2847087	+	219	WP_001314326.1	CopG family transcriptional regulator	-
MW398_RS14990 (2847311)	2847311..2848240	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
MW398_RS14995 (2848237)	2848237..2848872	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
MW398_RS15000 (2848869)	2848869..2849771	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T242080 WP_000897302.1 NZ_CP095505:2845234-2845545 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T242080 NZ_CP095505:2845234-2845545 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT242080 WP_000356397.1 NZ_CP095505:2845546-2845836 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT242080 NZ_CP095505:2845546-2845836 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGTACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416