Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1472529..1473256 Replicon chromosome
Accession NZ_CP095505
Organism Escherichia coli strain GN02050

Toxin (Protein)


Gene name higB Uniprot ID A0A8E0MHE9
Locus tag MW398_RS08390 Protein ID WP_021524447.1
Coordinates 1472945..1473256 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MW398_RS08385 Protein ID WP_000126304.1
Coordinates 1472529..1472948 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW398_RS08370 (1468692) 1468692..1470944 - 2253 WP_021524446.1 carbamoyltransferase HypF -
MW398_RS08375 (1471097) 1471097..1471624 - 528 WP_001078777.1 electron transport protein HydN -
MW398_RS08380 (1472009) 1472009..1472437 + 429 WP_000536064.1 hypothetical protein -
MW398_RS08385 (1472529) 1472529..1472948 - 420 WP_000126304.1 helix-turn-helix domain-containing protein Antitoxin
MW398_RS08390 (1472945) 1472945..1473256 - 312 WP_021524447.1 type II toxin-antitoxin system HigB family toxin Toxin
MW398_RS08395 (1473419) 1473419..1474174 - 756 WP_001314074.1 hypothetical protein -
MW398_RS08400 (1474217) 1474217..1474687 - 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
MW398_RS08405 (1474680) 1474680..1475090 - 411 WP_001291913.1 formate hydrogenlyase assembly protein HycH -
MW398_RS08410 (1475087) 1475087..1475854 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
MW398_RS08415 (1475854) 1475854..1476396 - 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
MW398_RS08420 (1476406) 1476406..1478115 - 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12428.24 Da        Isoelectric Point: 9.7074

>T242077 WP_021524447.1 NZ_CP095505:c1473256-1472945 [Escherichia coli]
MHIISKAPFEECARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNKLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T242077 NZ_OV408202:c4821898-4821614 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15474.50 Da        Isoelectric Point: 4.4596

>AT242077 WP_000126304.1 NZ_CP095505:c1472948-1472529 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT242077 NZ_OV408202:c4822130-4821888 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGGTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References