Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 6221..6783 Replicon chromosome
Accession NZ_CP095505
Organism Escherichia coli strain GN02050

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag MW398_RS00910 Protein ID WP_000605675.1
Coordinates 6505..6783 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag MW398_RS00905 Protein ID WP_000781370.1
Coordinates 6221..6505 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW398_RS00890 (1402) 1402..4449 + 3048 WP_077634457.1 formate dehydrogenase-N subunit alpha -
MW398_RS00895 (4462) 4462..5346 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
MW398_RS00900 (5339) 5339..5992 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
MW398_RS00905 (6221) 6221..6505 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
MW398_RS00910 (6505) 6505..6783 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MW398_RS00915 (6969) 6969..7979 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
MW398_RS00920 (8113) 8113..9810 - 1698 WP_000433464.1 malate dehydrogenase -
MW398_RS00925 (9967) 9967..10104 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
MW398_RS00930 (10206) 10206..10421 - 216 WP_000495766.1 biofilm-dependent modulation protein -
MW398_RS00935 (10766) 10766..11197 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T242066 WP_000605675.1 NZ_CP095505:c6783-6505 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T242066 NZ_OV350343:368118-368417 [Halomonas sp. TD01]
ATGCCCAGGTTCCGTATCACACCGAGAGCAAGAGACGATTTAAAAGATATCGGTCGCTATACGGAATGGCAGTGGGGCAA
GAACCAACGGAATACCTATCTAAGGGATTTTGAAAAACGGTTTCACTGGCTCGCAGAAAATCCCCTGCTAGGAAAACATC
GCTCGGATATCAGCGAGGGTTATTACAGCTACCCCCAGGGCCAGCACGTTATCTTTTACCTGATTGGCCAAGGGTGCATT
GAGATAATCGGCATTCCTCATAAAGAGATGGCTATCGTCTCTTATTTTCTGCCTGGTTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT242066 WP_000781370.1 NZ_CP095505:c6505-6221 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT242066 NZ_OV350343:367870-368118 [Halomonas sp. TD01]
ATGGCAACGACCAGTCTTAGCCTAGGGGAGCACTGGGAAGTCTTTATCAAAAACGAAGTCGCCAGTGGCCGCTACGGCTC
CGCAAGTGAGGTTGTGCGTGATGCGCTACGCTCCATGGAGGAACGCAAAAGTAAACTTGAGGCGCTACGTACACATCTAG
CCCAAGGTGCCAAGCAGGCTAGCGCCGGTGAATTCGTGGACGATTTCTCAATGGATGCATTAATCAACGACTTGGATAGC
GAAACGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References