Detailed information of TA system TA242051

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3822179..3822768
• Replicon: chromosome
• Accession: NZ_CP095475
• Organism: Pseudoxanthomonas winnipegensis strain ZSY-33

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MWN52_RS16845
• Protein ID: WP_286575606.1
• Coordinates: 3822487..3822768 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MWN52_RS16840
• Protein ID: WP_130525087.1
• Coordinates: 3822179..3822475 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWN52_RS16815 (MWN52_16835)	3817333..3818793	-	1461	WP_130525069.1	sensor histidine kinase	-
MWN52_RS16820 (MWN52_16840)	3818793..3819476	-	684	WP_130516330.1	response regulator transcription factor	-
MWN52_RS16825 (MWN52_16845)	3819552..3819923	-	372	WP_286575603.1	hypothetical protein	-
MWN52_RS16830 (MWN52_16850)	3820071..3820982	-	912	WP_286575604.1	arginine deiminase-related protein	-
MWN52_RS16835 (MWN52_16855)	3820996..3822003	-	1008	WP_286575605.1	tRNA dihydrouridine(20/20a) synthase DusA	-
MWN52_RS16840 (MWN52_16860)	3822179..3822475	-	297	WP_130525087.1	HigA family addiction module antitoxin	Antitoxin
MWN52_RS16845 (MWN52_16865)	3822487..3822768	-	282	WP_286575606.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MWN52_RS16850 (MWN52_16870)	3822861..3823322	-	462	WP_286575607.1	hypothetical protein	-
MWN52_RS16855 (MWN52_16875)	3823319..3824044	-	726	WP_130516316.1	HAD family hydrolase	-
MWN52_RS16860 (MWN52_16880)	3824177..3824437	-	261	WP_130516314.1	hypothetical protein	-
MWN52_RS16865 (MWN52_16885)	3824575..3824970	-	396	WP_286575608.1	EF-hand domain-containing protein	-
MWN52_RS16870 (MWN52_16890)	3825199..3825570	-	372	WP_286575609.1	hypothetical protein	-
MWN52_RS16875 (MWN52_16895)	3825625..3825813	-	189	WP_130525092.1	CPXCG motif-containing cysteine-rich protein	-
MWN52_RS16880 (MWN52_16900)	3825824..3826369	-	546	WP_131659577.1	hypothetical protein	-
MWN52_RS16885 (MWN52_16905)	3826577..3827722	+	1146	WP_286575610.1	PQQ-dependent sugar dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10732.32 Da
• Isoelectric Point: 9.9110

>T242051 WP_286575606.1 NZ_CP095475:c3822768-3822487 [Pseudoxanthomonas winnipegensis]
VAIQSFADKHTQHLFEGRRVRRWINVEVVAMRKLAMLNAAAVLDDLRIPPANRLEALQGDRRGQHSIRINDQYRVCFVWT
PTGPANVEIVDYH

Nucleotide

• Length: 282 bp

>T242051 NZ_OV049808:2519225-2519593 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGACAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10767.48 Da
• Isoelectric Point: 9.5302

>AT242051 WP_130525087.1 NZ_CP095475:c3822475-3822179 [Pseudoxanthomonas winnipegensis]
MRTIAYPKPGEILLHEFLEPMGITQYRLAKAIGVPQRRIGEIVGGNRGVTADTALRLSRYFGTSVGFWLGLQMDHDAAVA
RDALAETLAKIEPYRPAA

Nucleotide

• Length: 297 bp

>AT242051 NZ_OV049808:2519007-2519228 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA