Detailed information of TA system TA242037

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 74352..74778
• Replicon: plasmid pLS45-1
• Accession: NZ_CP095449
• Organism: Escherichia coli strain LS45

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MWN28_RS25250
• Protein ID: WP_001372321.1
• Coordinates: 74352..74477 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	74554..74778 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWN28_RS25205 (69402)	69402..69629	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
MWN28_RS25210 (69723)	69723..70407	-	685	Protein_81	PAS domain-containing protein	-
MWN28_RS25215 (70598)	70598..70981	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
MWN28_RS25220 (71258)	71258..71905	+	648	WP_000614936.1	transglycosylase SLT domain-containing protein	-
MWN28_RS25225 (72202)	72202..73024	-	823	Protein_84	DUF932 domain-containing protein	-
MWN28_RS25230 (73144)	73144..73430	-	287	Protein_85	hypothetical protein	-
MWN28_RS25235 (73455)	73455..73661	-	207	WP_000547939.1	hypothetical protein	-
MWN28_RS25240 (73731)	73731..73904	+	174	Protein_87	hypothetical protein	-
MWN28_RS25245 (73902)	73902..74132	-	231	WP_001426396.1	hypothetical protein	-
MWN28_RS25250 (74352)	74352..74477	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
MWN28_RS25255 (74419)	74419..74568	-	150	Protein_90	plasmid maintenance protein Mok	-
- (74554)	74554..74778	-	225	NuclAT_0	-	Antitoxin
- (74554)	74554..74778	-	225	NuclAT_0	-	Antitoxin
- (74554)	74554..74778	-	225	NuclAT_0	-	Antitoxin
- (74554)	74554..74778	-	225	NuclAT_0	-	Antitoxin
MWN28_RS25260 (74590)	74590..74778	+	189	WP_001299721.1	hypothetical protein	-
MWN28_RS25265 (74747)	74747..75314	-	568	Protein_92	plasmid SOS inhibition protein A	-
MWN28_RS25270 (75400)	75400..76146	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
MWN28_RS25275 (76161)	76161..77702	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
MWN28_RS25280 (77892)	77892..78095	-	204	Protein_95	plasmid SOS inhibition protein A	-
MWN28_RS25285 (78092)	78092..78525	-	434	Protein_96	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T242037 WP_001372321.1 NZ_CP095449:c74477-74352 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T242037 NZ_OV040584:569876-570154 [Haemophilus influenzae]
ATGTACGCGATTGCTTTTGATTTAGTTGTAAAAGACACTCAAGATGATCATCCAAAGGGCGTTCAAGAGGCTTATACAGA
CATTGGCGCAGTTTTAGCAAAATTTGGTTTTGTTAGAACACAAGGAAGTTTATATACCAACATGAATGAAGATATGGCGA
ATCTCTTTCAGGCAATGAATGCACTAAAACAGTTGGCGTGGATTTCTCAGTCGGTACGAGATATTCGCGCTTTTCGTATT
GAGCAATGGTCTGATTTTACTGATTTTATACGAAATTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT242037 NZ_CP095449:c74778-74554 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin