Detailed information of TA system TA024203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4075361..4076160
• Replicon: chromosome
• Accession: NC_013008
• Organism: Escherichia coli O157:H7 str. TW14359

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: ECSP_RS21325
• Protein ID: WP_000347273.1
• Coordinates: 4075696..4076160 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: ECSP_RS21320
• Protein ID: WP_001302819.1
• Coordinates: 4075361..4075696 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSP_RS21305	4071146..4071916	-	771	WP_001058231.1	2-dehydro-3-deoxyglucarate aldolase	-
ECSP_RS21310	4071932..4073266	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
ECSP_RS21315	4073641..4075212	+	1572	WP_001273776.1	galactarate dehydratase	-
ECSP_RS21320	4075361..4075696	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
ECSP_RS21325	4075696..4076160	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
ECSP_RS21330	4076215..4077024	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
ECSP_RS21335	4077273..4078553	+	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
ECSP_RS21340	4078576..4079049	+	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
ECSP_RS21345	4079060..4079839	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
ECSP_RS21350	4079829..4080707	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
ECSP_RS21355	4080725..4081159	+	435	WP_000948831.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T24203 WP_000347273.1 NC_013008:4075696-4076160 [Escherichia coli O157:H7 str. TW14359]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T24203 NC_013008:4075696-4076160 [Escherichia coli O157:H7 str. TW14359]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT24203 WP_001302819.1 NC_013008:4075361-4075696 [Escherichia coli O157:H7 str. TW14359]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT24203 NC_013008:4075361-4075696 [Escherichia coli O157:H7 str. TW14359]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7