Detailed information of TA system TA241999

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 20475..20728
• Replicon: plasmid pIM007_KPC
• Accession: NZ_CP095431
• Organism: Klebsiella pneumoniae strain IM007

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: MWR71_RS29810
• Protein ID: WP_001312851.1
• Coordinates: 20475..20624 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	20669..20728 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWR71_RS29775 (15834)	15834..16249	-	416	Protein_18	IS1-like element IS1B family transposase	-
MWR71_RS29780 (16498)	16498..16899	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
MWR71_RS29785 (16832)	16832..17089	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
MWR71_RS29790 (17182)	17182..17835	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
MWR71_RS29795 (18774)	18774..19631	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
MWR71_RS29800 (19624)	19624..19698	-	75	WP_001375168.1	RepA leader peptide Tap	-
MWR71_RS29805 (19943)	19943..20191	-	249	WP_000083837.1	replication regulatory protein RepA	-
MWR71_RS29810 (20475)	20475..20624	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (20669)	20669..20728	+	60	NuclAT_0	-	Antitoxin
- (20669)	20669..20728	+	60	NuclAT_0	-	Antitoxin
- (20669)	20669..20728	+	60	NuclAT_0	-	Antitoxin
- (20669)	20669..20728	+	60	NuclAT_0	-	Antitoxin
MWR71_RS29815 (20929)	20929..21261	-	333	WP_152916585.1	hypothetical protein	-
MWR71_RS29820 (21323)	21323..21922	-	600	WP_032083981.1	PIN domain-containing protein	-
MWR71_RS29825 (22308)	22308..22508	-	201	WP_015059022.1	hypothetical protein	-
MWR71_RS29830 (22640)	22640..23200	-	561	WP_000139328.1	fertility inhibition protein FinO	-
MWR71_RS29835 (23255)	23255..24001	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
MWR71_RS29840 (24021)	24021..24221	-	201	WP_072354025.1	hypothetical protein	-
MWR71_RS29845 (24246)	24246..24950	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
MWR71_RS29850 (25005)	25005..25370	-	366	Protein_33	type IV conjugative transfer system protein TraE	-
MWR71_RS29855 (25390)	25390..25695	-	306	WP_004144424.1	type IV conjugative transfer system protein TraL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaSHV-182 / blaCTX-M-65 / blaKPC-2 / blaSHV-12	-	1..72069	72069
	-	flank	IS/Tn	-	-	24246..24950	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T241999 WP_001312851.1 NZ_CP095431:c20624-20475 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T241999 NZ_OU943336:c4025441-4025157 [Salmonella enterica subsp. enterica serovar Typhi]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Nucleotide

• Length: 60 bp

>AT241999 NZ_CP095431:20669-20728 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin