Detailed information of TA system TA241996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 60632..61359
• Replicon: plasmid pIM007_vir
• Accession: NZ_CP095429
• Organism: Klebsiella pneumoniae strain IM007

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: MWR71_RS28030
• Protein ID: WP_011251285.1
• Coordinates: 60632..60943 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MWR71_RS28035
• Protein ID: WP_011251286.1
• Coordinates: 60940..61359 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWR71_RS27990 (MWR71_27945)	55643..56137	+	495	WP_011251274.1	hypothetical protein	-
MWR71_RS27995 (MWR71_27950)	56143..56583	+	441	WP_011251275.1	hypothetical protein	-
MWR71_RS28000 (MWR71_27955)	57008..57964	-	957	WP_011251280.1	DsbA family protein	-
MWR71_RS28005 (MWR71_27960)	58024..58365	-	342	WP_011251281.1	hypothetical protein	-
MWR71_RS28010 (MWR71_27965)	58379..58690	-	312	WP_011251282.1	hypothetical protein	-
MWR71_RS28015 (MWR71_27970)	58707..59156	-	450	WP_011251283.1	thioredoxin fold domain-containing protein	-
MWR71_RS28025 (MWR71_27980)	59990..60427	+	438	Protein_67	DDE-type integrase/transposase/recombinase	-
MWR71_RS28030 (MWR71_27985)	60632..60943	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
MWR71_RS28035 (MWR71_27990)	60940..61359	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
MWR71_RS28040 (MWR71_27995)	61506..62474	+	969	WP_074428168.1	IS5 family transposase	-
MWR71_RS28045 (MWR71_28000)	62546..62911	-	366	WP_048333448.1	hypothetical protein	-
MWR71_RS28050 (MWR71_28005)	62925..63713	-	789	WP_040217257.1	hypothetical protein	-
MWR71_RS28055 (MWR71_28010)	63734..64354	-	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
MWR71_RS28060 (MWR71_28015)	64773..65408	+	636	WP_223171879.1	hypothetical protein	-
MWR71_RS28065 (MWR71_28020)	65721..66191	+	471	WP_048333570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iutA / iroD / iroC / iroB / rmpA2 / iucA / iucB / iucC / iucD / iutA / iroD / iroC	1..228102	228102
	-	inside	IScluster/Tn	-	-	53723..71480	17757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T241996 WP_011251285.1 NZ_CP095429:60632-60943 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T241996 NZ_OU943336:1974652-1974754 [Salmonella enterica subsp. enterica serovar Typhi]
GCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT241996 WP_011251286.1 NZ_CP095429:60940-61359 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT241996 NZ_OU943336:c1974756-1974613 [Salmonella enterica subsp. enterica serovar Typhi]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCTCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1