Detailed information of TA system TA241972

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 35248..35501
• Replicon: plasmid pIM029_KPC
• Accession: NZ_CP095427
• Organism: Klebsiella pneumoniae strain IM029

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: MWR69_RS28125
• Protein ID: WP_001312851.1
• Coordinates: 35248..35397 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	35442..35501 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWR69_RS28090 (30607)	30607..31022	-	416	Protein_43	IS1 family transposase	-
MWR69_RS28095 (31271)	31271..31672	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
MWR69_RS28100 (31605)	31605..31862	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
MWR69_RS28105 (31955)	31955..32608	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
MWR69_RS28110 (33547)	33547..34404	-	858	WP_142295464.1	incFII family plasmid replication initiator RepA	-
MWR69_RS28115 (34397)	34397..34471	-	75	WP_001375168.1	RepA leader peptide Tap	-
MWR69_RS28120 (34716)	34716..34964	-	249	WP_000083837.1	replication regulatory protein RepA	-
MWR69_RS28125 (35248)	35248..35397	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (35442)	35442..35501	+	60	NuclAT_1	-	Antitoxin
- (35442)	35442..35501	+	60	NuclAT_1	-	Antitoxin
- (35442)	35442..35501	+	60	NuclAT_1	-	Antitoxin
- (35442)	35442..35501	+	60	NuclAT_1	-	Antitoxin
MWR69_RS28130 (35702)	35702..36034	-	333	WP_152916585.1	hypothetical protein	-
MWR69_RS28135 (36096)	36096..36695	-	600	WP_032083981.1	PIN domain-containing protein	-
MWR69_RS28140 (37081)	37081..37281	-	201	WP_015059022.1	hypothetical protein	-
MWR69_RS28145 (37413)	37413..37973	-	561	WP_000139328.1	fertility inhibition protein FinO	-
MWR69_RS28150 (38028)	38028..38774	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
MWR69_RS28155 (38794)	38794..38994	-	201	WP_072354025.1	hypothetical protein	-
MWR69_RS28160 (39019)	39019..39723	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
MWR69_RS28165 (39776)	39776..39841	+	66	Protein_58	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	rmtB / fosA3 / blaKPC-2 / blaSHV-12	-	1..120647	120647

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T241972 WP_001312851.1 NZ_CP095427:c35397-35248 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T241972 NZ_OU745390:2877019-2877321 [Pseudomonas sp. Marseille-Q3773]
ATGAAAAAAATCGAATCCAGCAGTTTCAGACATTGGGTGACGGGTCTCCGAGACGCTAATGCCAGGGCCCGGATCATTTC
TCGGATCAACCGGTTGATGGAGGGCCTGCCTGGCGACGTATCACCGGTTGGCCACGGCGTCAGTGAGCTCAGGGTGCACT
ATGGGCCCGGTTACCGGGTGTACTTCCATCAGGCTGGCAACACGTTTGTCATTCTGCTTTGCGGCGGCGACAAAGGCAGC
CAACAGCGGGACATCAAGGCAGCCCACCAGATTCTGCGTTCGTGGAGGATGCAAAATGACTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT241972 NZ_CP095427:35442-35501 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin