Detailed information of TA system TA241969

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 7142..7568
• Replicon: plasmid pIM029_KPC
• Accession: NZ_CP095427
• Organism: Klebsiella pneumoniae strain IM029

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MWR69_RS27935
• Protein ID: WP_001372321.1
• Coordinates: 7142..7267 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	7344..7568 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWR69_RS27900 (2197)	2197..2424	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
MWR69_RS27905 (2518)	2518..3204	-	687	WP_015059009.1	PAS domain-containing protein	-
MWR69_RS27910 (3395)	3395..3778	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
MWR69_RS27915 (4055)	4055..4702	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
MWR69_RS27920 (4999)	4999..5820	-	822	WP_001234445.1	DUF932 domain-containing protein	-
MWR69_RS27925 (5931)	5931..6227	-	297	WP_001272251.1	hypothetical protein	-
MWR69_RS27930 (6527)	6527..6823	+	297	Protein_11	hypothetical protein	-
MWR69_RS27935 (7142)	7142..7267	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
MWR69_RS27940 (7209)	7209..7358	-	150	Protein_13	plasmid maintenance protein Mok	-
- (7344)	7344..7568	-	225	NuclAT_0	-	Antitoxin
- (7344)	7344..7568	-	225	NuclAT_0	-	Antitoxin
- (7344)	7344..7568	-	225	NuclAT_0	-	Antitoxin
- (7344)	7344..7568	-	225	NuclAT_0	-	Antitoxin
MWR69_RS27945 (7580)	7580..8299	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
MWR69_RS27950 (8296)	8296..8730	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
MWR69_RS27955 (8799)	8799..10822	-	2024	Protein_16	ParB/RepB/Spo0J family partition protein	-
MWR69_RS27960 (10883)	10883..11116	-	234	WP_000006003.1	DUF905 family protein	-
MWR69_RS27965 (11174)	11174..11701	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
MWR69_RS27970 (12003)	12003..12458	+	456	Protein_19	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	rmtB / fosA3 / blaKPC-2 / blaSHV-12	-	1..120647	120647

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T241969 WP_001372321.1 NZ_CP095427:c7267-7142 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T241969 NZ_OU745373:1369639-1369935 [Cardiobacterium sp. Marseille-Q4385]
ATGAATTACCGATTCGAATGGGATGATGGAAAAGAGCGCATCAATATCCGTAAACACAGCATAGATTTCACAATGGCCTC
CACCGTTTTCCTTGATCCGCTACATCAAATGGTGCAAGACCGCATAGAAGGTGGCGAGTGGCGATGGCAGACTATAGGAA
TCGTTAAAGGGCAAGTCGTTGTTTTGGTTGCTCATACTGTTGTAGAGACAGATACAATGACCTGCATCCGCATTATCTCG
GCACGCCATGCCACCAAAAAAGAACAGGAGTATTACTATGGGCGTACTTATCATTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT241969 NZ_CP095427:c7568-7344 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin