Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 356616..357262 Replicon chromosome
Accession NZ_CP095426
Organism Klebsiella pneumoniae strain IM029

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag MWR69_RS01645 Protein ID WP_002920560.1
Coordinates 356616..356963 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag MWR69_RS01650 Protein ID WP_002920557.1
Coordinates 356963..357262 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWR69_RS01635 (MWR69_01635) 352542..353975 + 1434 WP_002920564.1 glycogen synthase GlgA -
MWR69_RS01640 (MWR69_01640) 353993..356440 + 2448 WP_002920561.1 glycogen phosphorylase -
MWR69_RS01645 (MWR69_01645) 356616..356963 + 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MWR69_RS01650 (MWR69_01650) 356963..357262 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
MWR69_RS01655 (MWR69_01655) 357325..358833 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
MWR69_RS01660 (MWR69_01660) 359038..359367 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
MWR69_RS01665 (MWR69_01665) 359418..360248 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
MWR69_RS01670 (MWR69_01670) 360298..361056 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T241950 WP_002920560.1 NZ_CP095426:356616-356963 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T241950 NZ_OU701452:c2060729-2060626 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT241950 WP_002920557.1 NZ_CP095426:356963-357262 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT241950 NZ_OU701452:2060588-2060859 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References