Detailed information of TA system TA241940

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30023..30449
• Replicon: plasmid pIM057_KPC
• Accession: NZ_CP095423
• Organism: Klebsiella pneumoniae strain IM057

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MWR70_RS28560
• Protein ID: WP_001372321.1
• Coordinates: 30023..30148 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	30225..30449 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWR70_RS28525 (25078)	25078..25305	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
MWR70_RS28530 (25399)	25399..26085	-	687	WP_015059009.1	PAS domain-containing protein	-
MWR70_RS28535 (26276)	26276..26659	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
MWR70_RS28540 (26936)	26936..27583	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
MWR70_RS28545 (27880)	27880..28701	-	822	WP_001234445.1	DUF932 domain-containing protein	-
MWR70_RS28550 (28812)	28812..29108	-	297	WP_001272251.1	hypothetical protein	-
MWR70_RS28555 (29408)	29408..29704	+	297	Protein_40	hypothetical protein	-
MWR70_RS28560 (30023)	30023..30148	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
MWR70_RS28565 (30090)	30090..30239	-	150	Protein_42	plasmid maintenance protein Mok	-
- (30225)	30225..30449	-	225	NuclAT_0	-	Antitoxin
- (30225)	30225..30449	-	225	NuclAT_0	-	Antitoxin
- (30225)	30225..30449	-	225	NuclAT_0	-	Antitoxin
- (30225)	30225..30449	-	225	NuclAT_0	-	Antitoxin
MWR70_RS28570 (30461)	30461..31180	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
MWR70_RS28575 (31177)	31177..31611	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
MWR70_RS28580 (31680)	31680..33703	-	2024	Protein_45	ParB/RepB/Spo0J family partition protein	-
MWR70_RS28585 (33764)	33764..33997	-	234	WP_000006003.1	DUF905 family protein	-
MWR70_RS28590 (34055)	34055..34582	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
MWR70_RS28595 (34884)	34884..35345	+	462	Protein_48	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / blaCTX-M-65 / catA2	-	1..129505	129505

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T241940 WP_001372321.1 NZ_CP095423:c30148-30023 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T241940 NZ_OU701452:284904-285302 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT241940 NZ_CP095423:c30449-30225 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin