Detailed information of TA system TA241901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-DinJ
• Location: 496291..496776
• Replicon: chromosome
• Accession: NZ_CP095384
• Organism: Lacticaseibacillus rhamnosus strain VHProbi M14

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: MU539_RS02360
• Protein ID: WP_080741008.1
• Coordinates: 496291..496476 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: K8QJE7
• Locus tag: MU539_RS02365
• Protein ID: WP_005714994.1
• Coordinates: 496504..496776 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU539_RS02335 (MU539_02325)	491308..491997	+	690	WP_033572239.1	rhomboid family intramembrane serine protease	-
MU539_RS02340 (MU539_02330)	492478..492633	-	156	WP_235717767.1	hypothetical protein	-
MU539_RS02345 (MU539_02335)	493536..493934	+	399	WP_235717768.1	hypothetical protein	-
MU539_RS02350 (MU539_02340)	493921..494409	+	489	WP_235717769.1	hypothetical protein	-
MU539_RS02355 (MU539_02345)	494652..496067	-	1416	WP_033572240.1	3'-5' exonuclease	-
MU539_RS02360 (MU539_02350)	496291..496476	-	186	WP_080741008.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
MU539_RS02365 (MU539_02355)	496504..496776	-	273	WP_005714994.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
MU539_RS02370 (MU539_02360)	496889..497182	+	294	WP_005684269.1	metalloregulator ArsR/SmtB family transcription factor	-
MU539_RS02375 (MU539_02365)	497264..498427	+	1164	WP_005714995.1	MFS transporter	-
MU539_RS02380 (MU539_02370)	498581..499156	+	576	WP_005714996.1	DNA-3-methyladenine glycosylase I	-
MU539_RS02385 (MU539_02375)	499278..499496	+	219	WP_032960261.1	hypothetical protein	-
MU539_RS02390 (MU539_02380)	499623..500504	-	882	WP_005684263.1	class II fructose-1,6-bisphosphate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (11-56)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-61)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6760.85 Da
• Isoelectric Point: 7.5258

>T241901 WP_080741008.1 NZ_CP095384:c496476-496291 [Lacticaseibacillus rhamnosus]
MQRQAHVKLASLHGHALRGAHSGERALHIAPDWLLVYKVDAEALILMLLATGTHRDTLNIE

Nucleotide

• Length: 186 bp

>T241901 NZ_OU659164:44970-45248 [Providencia alcalifaciens]
ATGGAATTAAAATGGACGAGTAAGGCATTATCGGATCTTTCTCGTGTATATGATTTTTTAGCGTTATCAAACTCAGTCGC
TGCCGCGCGTGTTGTGCAAACATTGACGAAAGCCCCCATGTTGTTGTTGATAAAAAACCCCCGAATGGGAGAGCAATTGT
TTCAATTTATGCCACACGAAGTCCGTCGGATTGTGGTGGTGAATTATGAGATACGTTATGAACTCATTGATTCGACAATC
TATATTTTACGGATATGGCATACACGAGAAGATCGGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10280.92 Da
• Isoelectric Point: 10.4652

>AT241901 WP_005714994.1 NZ_CP095384:c496776-496504 [Lacticaseibacillus rhamnosus]
MAKESRIRNWINTITKKRALHVLNRLGLERFSAINMYSKRIGDTGALPFTLEMSFADQLRFAEADVKAGRIKSFKTVGAL
MKDLYNDVDD

Nucleotide

• Length: 273 bp

>AT241901 NZ_OU659164:44689-44979 [Providencia alcalifaciens]
ATGAAAAACAGCTCGGTACAAAATAAGACAAAAGTGCTAACAGCACATGTGCCTTTAACGATGGCAGATAAGATTGATGA
AATAGCCGAACGTCTTGAACGTTCCCGTGGATGGGTCATGAAGCAAGCGTTGTCTACGTGGATAGCTCAAGAGGAGATGC
GGGACACATTGACACGAGAAGCGTTATTGGATGTTGAATCTGGCCAAGTGATAGATAATCAGTCTGTGCAAGATTGGGCG
GCTAGCTTGAATACTGAACTACCATTACCGGTGCCACGCTGATGGAATTAA

Structures

Antitoxin

• AlphaFold DB: K8QJE7