Detailed information of TA system TA024189

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2100929..2101154
• Replicon: chromosome
• Accession: NC_013008
• Organism: Escherichia coli O157:H7 str. TW14359

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ECSP_RS10720
• Protein ID: WP_000813258.1
• Coordinates: 2100929..2101084 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2101096..2101154 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSP_RS10670	2095932..2096363	-	432	WP_001302123.1	tellurite resistance protein	-
ECSP_RS10685	2096814..2097527	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ECSP_RS33080	2097663..2097860	-	198	WP_000917763.1	hypothetical protein	-
ECSP_RS10695	2098085..2098639	-	555	WP_000640035.1	DUF1133 family protein	-
ECSP_RS10700	2098702..2099007	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ECSP_RS10705	2099020..2100069	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
ECSP_RS10710	2100071..2100343	-	273	WP_000191872.1	hypothetical protein	-
ECSP_RS10715	2100465..2100809	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ECSP_RS10720	2100929..2101084	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2101096..2101154	+	59	-	-	Antitoxin
ECSP_RS10725	2101375..2101932	-	558	WP_000104474.1	DUF551 domain-containing protein	-
ECSP_RS10730	2101934..2102152	-	219	WP_000683609.1	DUF4014 family protein	-
ECSP_RS10735	2102280..2102591	-	312	WP_001289673.1	hypothetical protein	-
ECSP_RS10740	2102584..2102811	-	228	WP_000699809.1	hypothetical protein	-
ECSP_RS10745	2102808..2103089	-	282	WP_000603384.1	DNA-binding protein	-
ECSP_RS10750	2103122..2103838	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
ECSP_RS33085	2103872..2104333	-	462	WP_000139447.1	replication protein	-
ECSP_RS33090	2104326..2105369	-	1044	WP_001262402.1	hypothetical protein	-
ECSP_RS10765	2105438..2105863	-	426	WP_000693878.1	Rha family transcriptional regulator	-
ECSP_RS10770	2105847..2106089	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2070475..2162948	92473

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T24189 WP_000813258.1 NC_013008:c2101084-2100929 [Escherichia coli O157:H7 str. TW14359]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T24189 NC_013008:c2101084-2100929 [Escherichia coli O157:H7 str. TW14359]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT24189 NC_013008:2101096-2101154 [Escherichia coli O157:H7 str. TW14359]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin