Detailed information of TA system TA241873

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1181953..1182680
• Replicon: chromosome
• Accession: NZ_CP095276
• Organism: Enterobacter hormaechei subsp. xiangfangensis strain HD8642

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MU851_RS05530
• Protein ID: WP_045348581.1
• Coordinates: 1182369..1182680 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8B3V464
• Locus tag: MU851_RS05525
• Protein ID: WP_022650546.1
• Coordinates: 1181953..1182372 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU851_RS05515 (MU851_05505)	1179783..1180904	-	1122	WP_015571201.1	YbdK family carboxylate-amine ligase	-
MU851_RS05520 (MU851_05510)	1181010..1181900	+	891	WP_063136467.1	oxidoreductase	-
MU851_RS05525 (MU851_05515)	1181953..1182372	-	420	WP_022650546.1	helix-turn-helix domain-containing protein	Antitoxin
MU851_RS05530 (MU851_05520)	1182369..1182680	-	312	WP_045348581.1	type II toxin-antitoxin system HigB family toxin	Toxin
MU851_RS05535 (MU851_05525)	1183052..1183648	+	597	WP_024471104.1	hypothetical protein	-
MU851_RS05540 (MU851_05530)	1183620..1183847	+	228	WP_150317918.1	hypothetical protein	-
MU851_RS05545 (MU851_05535)	1184240..1185157	+	918	WP_150317919.1	hypothetical protein	-
MU851_RS05550 (MU851_05540)	1185324..1185926	+	603	WP_023320086.1	site-specific integrase	-
MU851_RS05555 (MU851_05545)	1186063..1186731	+	669	WP_023296073.1	epoxyqueuosine reductase QueH	-
MU851_RS05560 (MU851_05550)	1186955..1187149	-	195	WP_223825263.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-134)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12562.47 Da
• Isoelectric Point: 9.9435

>T241873 WP_045348581.1 NZ_CP095276:c1182680-1182369 [Enterobacter hormaechei subsp. xiangfangensis]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPLPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKI
FIKHISTHADYDKLMDYYRRTKE

Nucleotide

• Length: 312 bp

>T241873 NZ_OU659121:146913-147191 [Providencia alcalifaciens]
ATGATAAAATCGTTTAAACATAAAGGGTTGAAACAACTCTTTGAAAAAGGAATAACTTCAGGGGTTCCGGCTCAAGATGC
TGAACGGATCAACGACCGTTTACAAGCAATAGATATCGCTAACGATATTCGTGAATTAGACCGCCAAATTTACAAATTGC
ATCCACTGAAAGGTGACAGAGAAGGTTATTGGTCAATAACAGTGCGAGCGAATTGGCGCATTACTTTTCAATTCATCAAT
GGTGATGCTTACATCTTAAATTATGAGGATTATCACTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15447.51 Da
• Isoelectric Point: 4.6051

>AT241873 WP_022650546.1 NZ_CP095276:c1182372-1181953 [Enterobacter hormaechei subsp. xiangfangensis]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVEMLTAKIDKYENESPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Nucleotide

• Length: 420 bp

>AT241873 NZ_OU659121:147191-147505 [Providencia alcalifaciens]
ATGAGACAGTTTAAAATATCTCACCCAGGTGAAATTATTGCACGAGATTTGGCTGATATGGGGATCAGTGGTCGACGTTT
TGCATTAAATATTGGTGTAACGCCAGCAACAGTTTCCCGTTTTTTATCAGGTAAAACAGCATTGACTCCCGCATTGGCAA
TCCGTATCGCCGCTGCACTAGGAAGTACTCCTGAATTTTGGTTACGTTTACAAAGTGCTTATGATTTACGTCAATTAGAA
AATCAAATTGATACGTCCGATATTATTTTGTATGAAGAGGCGCCTGATAGCGCTAAAGGCATATCCAAACATTAG

Structures

Antitoxin

• AlphaFold DB: A0A8B3V464