Detailed information of TA system TA241867

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 98439..98693
• Replicon: plasmid pEC9682-1
• Accession: NZ_CP095272
• Organism: Escherichia coli strain EC9682

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: MU524_RS25825
• Protein ID: WP_001312851.1
• Coordinates: 98544..98693 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	98439..98500 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU524_RS25800 (95186)	95186..95932	+	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
MU524_RS25805 (95991)	95991..96851	+	861	WP_000704514.1	alpha/beta hydrolase	-
MU524_RS25810 (96954)	96954..97514	+	561	WP_000139315.1	fertility inhibition protein FinO	-
MU524_RS25815 (97650)	97650..97862	+	213	WP_001299730.1	ANR family transcriptional regulator	-
MU524_RS25820 (98108)	98108..98182	+	75	Protein_104	endonuclease	-
- (98439)	98439..98500	-	62	NuclAT_1	-	Antitoxin
- (98439)	98439..98500	-	62	NuclAT_1	-	Antitoxin
- (98439)	98439..98500	-	62	NuclAT_1	-	Antitoxin
- (98439)	98439..98500	-	62	NuclAT_1	-	Antitoxin
MU524_RS25825 (98544)	98544..98693	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
MU524_RS25830 (98977)	98977..99234	+	258	WP_000083845.1	replication regulatory protein RepA	-
MU524_RS25835 (99251)	99251..99502	-	252	WP_223195197.1	replication protein RepA	-
MU524_RS25840 (99493)	99493..99540	+	48	WP_229471593.1	hypothetical protein	-
MU524_RS25845 (99533)	99533..100015	+	483	WP_001273588.1	hypothetical protein	-
MU524_RS25850 (100008)	100008..100571	+	564	Protein_110	plasmid replication initiator RepA	-
MU524_RS25855 (100763)	100763..102304	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
MU524_RS25860 (102319)	102319..103065	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
MU524_RS25865 (103152)	103152..103451	+	300	Protein_113	incFII family plasmid replication initiator RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / aph(3'')-Ib / sul2 / tet(B) / catA1 / dfrA17	iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..146265	146265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T241867 WP_001312851.1 NZ_CP095272:98544-98693 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T241867 NZ_OU659118:3897883-3898143 [Providencia alcalifaciens]
ATGAGTAGACTTCTAACATGGACGGATCAAGCTTGGGAAGACTATCTATACTGGCAAAACCAAGATAAAAAGACATTAAA
GCGGATAAATAAACTCATTGAAGACACGAAAAGAACGCCATTCAGCGGTATAGGTAAACCAGAGCCACTAAAAGAAAACT
TGGCTGGCTTCTGGTCTCGTAGAATAGATGAAACAAACAGGCTAGTTTATGCAATCGAAAATACCGCATTAGTTATCATT
GCCTGCCGCTATCACTACTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT241867 NZ_CP095272:c98500-98439 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin