Detailed information of TA system TA241862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4955967..4956569
• Replicon: chromosome
• Accession: NZ_CP095271
• Organism: Escherichia coli strain EC9682

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: MU524_RS24275
• Protein ID: WP_000897302.1
• Coordinates: 4956258..4956569 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MU524_RS24270
• Protein ID: WP_000356397.1
• Coordinates: 4955967..4956257 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU524_RS24245 (4952040)	4952040..4952942	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
MU524_RS24250 (4952939)	4952939..4953574	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
MU524_RS24255 (4953571)	4953571..4954500	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
MU524_RS24260 (4954716)	4954716..4954934	-	219	WP_001314326.1	CopG family transcriptional regulator	-
MU524_RS24265 (4955330)	4955330..4955608	-	279	WP_001296612.1	hypothetical protein	-
MU524_RS24270 (4955967)	4955967..4956257	-	291	WP_000356397.1	NadS family protein	Antitoxin
MU524_RS24275 (4956258)	4956258..4956569	-	312	WP_000897302.1	hypothetical protein	Toxin
MU524_RS24280 (4956798)	4956798..4957706	+	909	WP_001331553.1	alpha/beta hydrolase	-
MU524_RS24285 (4957770)	4957770..4958711	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
MU524_RS24290 (4958756)	4958756..4959193	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
MU524_RS24295 (4959190)	4959190..4960062	-	873	WP_000920762.1	virulence factor BrkB family protein	-
MU524_RS24300 (4960056)	4960056..4960655	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T241862 WP_000897302.1 NZ_CP095271:c4956569-4956258 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T241862 NZ_OU659115:c66254-65976 [Providencia alcalifaciens]
ATGGAATTAAAATGGACGAGTAAGGCATTATCGGATCTTTCTCGTGTATATGATTTTTTAGCGTTATCAAACTCAGTCGC
TGCCGCGCGTGTTGTGCAAACATTGACGAAAGCCCCCATGTTGTTGTTGATAAAAAACCCCCGAATGGGAGAGCAATTGT
TTCAATTTATGCCACACGAAGTCCGTCGGATTGTGGTGGTGAATTATGAGATACGTTATGAACTCATTGATTCGACAATC
TATATTTTACGGATATGGCATACACGAGAAGATCGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT241862 WP_000356397.1 NZ_CP095271:c4956257-4955967 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT241862 NZ_OU659115:c65976-65833 [Providencia alcalifaciens]
ATGAGACAATTTAAAGTATCTCACCTTGGTGAAATTATCGCTAGAGACTTGGCTGATATGGGGATTAGTGGTCGACGTTT
CGCGCTCAATATTGATGTCACTCCAGCGACCGCTGGCAATTCGAATTGCTGCTGCATTAGATAG

Structures

Toxin

• AlphaFold DB: S1P416