Detailed information of TA system TA241857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3967652..3968346
• Replicon: chromosome
• Accession: NZ_CP095271
• Organism: Escherichia coli strain EC9682

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: MU524_RS19710
• Protein ID: WP_001263500.1
• Coordinates: 3967652..3968050 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: MU524_RS19715
• Protein ID: WP_000554758.1
• Coordinates: 3968053..3968346 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU524_RS19685 (3963017)	3963017..3963475	-	459	WP_001291988.1	xanthine phosphoribosyltransferase	-
MU524_RS19690 (3963736)	3963736..3965193	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
MU524_RS19695 (3965250)	3965250..3965864	-	615	WP_000602123.1	peptide chain release factor H	-
MU524_RS19700 (3965861)	3965861..3967000	-	1140	WP_000521577.1	RNA ligase RtcB family protein	-
MU524_RS19705 (3967190)	3967190..3967642	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
MU524_RS19710 (3967652)	3967652..3968050	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
MU524_RS19715 (3968053)	3968053..3968346	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
MU524_RS19720 (3968398)	3968398..3969453	-	1056	WP_001226168.1	DNA polymerase IV	-
MU524_RS19725 (3969524)	3969524..3970309	-	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
MU524_RS19730 (3970281)	3970281..3971993	+	1713	Protein_3867	flagellar biosynthesis protein FlhA	-
MU524_RS19735 (3972072)	3972072..3972230	+	159	WP_014639450.1	hypothetical protein	-
MU524_RS19740 (3972313)	3972313..3972810	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3967652..3988006	20354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T241857 WP_001263500.1 NZ_CP095271:c3968050-3967652 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T241857 NZ_OU659113:c853617-853414 [Providencia alcalifaciens]
ATGACCAAATCCGATTATCTGATGCGTTTAAGAAAATGCACCACTATTGAAACACTTGAGCGCGTTATTGAAAAAAATAA
GTATGAGTTATCCGATGATGAGTTAGAGTTATTTTACTCTGCCGCTGATCATCGTCTTGCAGAGCTCACGATGAATAAAC
TTTACGATAAAATTCCAGCATCTGTTTGGAAGTTTGTTAGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT241857 WP_000554758.1 NZ_CP095271:c3968346-3968053 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT241857 NZ_OU659113:c854058-853690 [Providencia alcalifaciens]
ATGGATGAATACTCACCCAAGAAACATGATATAGCCGAATTAAAATATCTTTGTAACAGTTTGAATAGAGACGCTATATC
AAGTTTACAAAAAACAAACACTCATTGGATAAATGATCTTAGTTCTGCACAAAGCATCAGTCTTAACGAGCTTGTTGAGC
ATATTGCAGCTTTTGTCTGGCGATTTAAAATCAAATATCCAAAAGAAAATCTCGTTATTTCTCTTGTTGAAGAGTATCTT
GACGAAACATATAATTTATTTGGCAGCCCAGTAGTTACCTTCAGCGAAATCATTGACTGGGAAAGTATGAATCAAAATCT
TGTCGCCGTGCTCGATGATGATCTAAAATGTCTGACAAGTAAGACTTAG

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3