Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3738740..3739419 Replicon chromosome
Accession NZ_CP095271
Organism Escherichia coli strain EC9682

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag MU524_RS18650 Protein ID WP_000057524.1
Coordinates 3739117..3739419 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag MU524_RS18645 Protein ID WP_000806442.1
Coordinates 3738740..3739081 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU524_RS18635 (3734984) 3734984..3735916 - 933 WP_000883052.1 glutaminase A -
MU524_RS18640 (3736178) 3736178..3738682 + 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
MU524_RS18645 (3738740) 3738740..3739081 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
MU524_RS18650 (3739117) 3739117..3739419 - 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MU524_RS18655 (3739552) 3739552..3740346 + 795 WP_000365177.1 TraB/GumN family protein -
MU524_RS18660 (3740550) 3740550..3741029 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
MU524_RS18665 (3741197) 3741197..3741853 + 657 WP_001363258.1 hypothetical protein -
MU524_RS18670 (3741850) 3741850..3742353 + 504 WP_000667000.1 hypothetical protein -
MU524_RS18675 (3742391) 3742391..3744043 - 1653 WP_000771762.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3731429..3742353 10924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T241855 WP_000057524.1 NZ_CP095271:c3739419-3739117 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T241855 NZ_OU594967:1545603-1545857 [Celerinatantimonas diazotrophica]
ATGGATGTAATGAGCTATTCACAATTTCGAGCGCAGTTAGCTAAAACGCTTGATGATGTGAACGATGACCACAAGCCGGT
GATGATCACTCGACAAAATGGTAAGCCTGCGATTGTCATGAGCGTTGAAGATTTCAAAGCGTATGAAGAGACTGCCTATC
TTATGGCGAGCCCTAAGAACGCACAGCGGCTGAACGCGGCCATTTCAGAACTAGAAGGTGGTCATGGCAGTGAAAAGGGG
CTGATTGAAGAATGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT241855 WP_000806442.1 NZ_CP095271:c3739081-3738740 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT241855 NZ_OU594967:1545854-1546108 [Celerinatantimonas diazotrophica]
ATGATGTTAGTGTGGGCATCCAGTGCCTGGGAAGATTATTTATATTGGCAGCAAACCGATAAAAAGATATTAAAACGTAT
CAATCAGCTCATTAAAGAAATTAGTCGAGAACCATTTGAAGGCATTGGTGATCCTGAGCCATTAAAACATCAATGGACAG
GCTATTGGTCTCGACGGATTAATAGAGAACATCGGCTTGTTTATAAAGCTAAAGAAAACGAAGTTTTGATTGCTCAATGT
CGGTATCACTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References