Detailed information of TA system TA241838

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hokW-rdlD/-
• Location: 2096916..2097141
• Replicon: chromosome
• Accession: NZ_CP095271
• Organism: Escherichia coli strain EC9682

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: MU524_RS10035
• Protein ID: WP_000813254.1
• Coordinates: 2096986..2097141 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	rdlD
Locus tag	-
Coordinates	2096916..2096974 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU524_RS09990	2092178..2092330	-	153	WP_000379609.1	DUF1391 family protein	-
MU524_RS09995	2092602..2092889	-	288	WP_000936799.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MU524_RS10000	2092889..2093080	-	192	WP_000100899.1	hypothetical protein	-
MU524_RS10005	2093108..2093509	-	402	WP_001329851.1	helix-turn-helix domain-containing protein	-
MU524_RS10010	2093619..2093891	+	273	WP_000887447.1	YdaS family helix-turn-helix protein	-
MU524_RS10015	2093875..2094300	+	426	WP_000693918.1	toxin YdaT family protein	-
MU524_RS10020	2094372..2095442	+	1071	WP_001262355.1	hypothetical protein	-
MU524_RS10025	2095483..2095908	+	426	WP_001151152.1	DUF977 family protein	-
MU524_RS10030	2096083..2096748	+	666	WP_000150294.1	epoxyqueuosine reductase QueH	-
-	2096916..2096974	-	59	-	-	Antitoxin
MU524_RS10035	2096986..2097141	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
MU524_RS10040	2097309..2097587	+	279	WP_024193993.1	hypothetical protein	-
MU524_RS10045	2097589..2098638	+	1050	WP_001265292.1	DUF968 domain-containing protein	-
MU524_RS10050	2098651..2099025	+	375	WP_001217427.1	RusA family crossover junction endodeoxyribonuclease	-
MU524_RS10055	2099022..2099843	+	822	WP_001235237.1	antitermination protein	-
MU524_RS10060	2100089..2100802	+	714	WP_000342820.1	hypothetical protein	-
MU524_RS10085	2101527..2101742	+	216	WP_000839572.1	class II holin family protein	-
MU524_RS10090	2101747..2102061	+	315	WP_000193273.1	YdfR family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ybtP / ybtQ / ybtX / ybtS	2076187..2130447	54260

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T241838 WP_000813254.1 NZ_CP095271:2096986-2097141 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T241838 NZ_OU342920:c11094-10945 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTTACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT241838 NZ_CP095271:c2096974-2096916 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin