Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1923135..1923637 Replicon chromosome
Accession NZ_CP095271
Organism Escherichia coli strain EC9682

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag MU524_RS09365 Protein ID WP_000767822.1
Coordinates 1923383..1923637 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag MU524_RS09360 Protein ID WP_001259255.1
Coordinates 1923135..1923386 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU524_RS09335 (1918313) 1918313..1919380 - 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
MU524_RS09340 (1919380) 1919380..1920450 - 1071 WP_000108995.1 histidinol-phosphate transaminase -
MU524_RS09345 (1920447) 1920447..1921751 - 1305 WP_001297912.1 histidinol dehydrogenase -
MU524_RS09350 (1921757) 1921757..1922656 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
MU524_RS09355 (1922802) 1922802..1922852 - 51 WP_001364200.1 his operon leader peptide -
MU524_RS09360 (1923135) 1923135..1923386 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
MU524_RS09365 (1923383) 1923383..1923637 + 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
MU524_RS09370 (1923720) 1923720..1924544 + 825 WP_000754733.1 SDR family oxidoreductase -
MU524_RS09375 (1924590) 1924590..1925519 + 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
MU524_RS09380 (1925734) 1925734..1925796 + 63 WP_010723108.1 membrane protein YoeI -
MU524_RS09385 (1925786) 1925786..1927144 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T241835 WP_000767822.1 NZ_CP095271:1923383-1923637 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T241835 NZ_OU342919:4450782-4450889 [Escherichia coli]
ATGACGCTCGCGCAGTTTACCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT241835 WP_001259255.1 NZ_CP095271:1923135-1923386 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT241835 NZ_OU342919:c4450735-4450669 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References