Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 1398009..1398804 | Replicon | chromosome |
| Accession | NC_013008 | ||
| Organism | Escherichia coli O157:H7 str. TW14359 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | B7UP43 |
| Locus tag | ECSP_RS06850 | Protein ID | WP_000854914.1 |
| Coordinates | 1398430..1398804 (+) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A0B0VS41 |
| Locus tag | ECSP_RS06845 | Protein ID | WP_001280954.1 |
| Coordinates | 1398009..1398383 (+) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ECSP_RS06805 | 1393364..1394269 | + | 906 | WP_000203541.1 | chemotaxis protein | - |
| ECSP_RS06810 | 1394266..1395336 | + | 1071 | WP_000102669.1 | patatin-like phospholipase family protein | - |
| ECSP_RS06820 | 1395676..1396494 | + | 819 | WP_001234682.1 | DUF945 domain-containing protein | - |
| ECSP_RS06825 | 1396585..1397070 | + | 486 | WP_000214398.1 | antirestriction protein | - |
| ECSP_RS06830 | 1397086..1397562 | + | 477 | WP_001186738.1 | RadC family protein | - |
| ECSP_RS06835 | 1397625..1397846 | + | 222 | WP_001220314.1 | DUF987 domain-containing protein | - |
| ECSP_RS06845 | 1398009..1398383 | + | 375 | WP_001280954.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| ECSP_RS06850 | 1398430..1398804 | + | 375 | WP_000854914.1 | TA system toxin CbtA family protein | Toxin |
| ECSP_RS06855 | 1398801..1399292 | + | 492 | WP_000976853.1 | hypothetical protein | - |
| ECSP_RS06860 | 1399304..1399501 | + | 198 | WP_000839282.1 | DUF957 domain-containing protein | - |
| ECSP_RS06865 | 1399586..1400428 | + | 843 | WP_001280481.1 | DUF4942 domain-containing protein | - |
| ECSP_RS06875 | 1400899..1401837 | + | 939 | WP_000351284.1 | glyoxylate/hydroxypyruvate reductase GhrA | - |
| ECSP_RS06880 | 1401892..1402629 | + | 738 | WP_000283664.1 | zinc-binding phosphatase | - |
| ECSP_RS06885 | 1402653..1403207 | + | 555 | WP_001001917.1 | molecular chaperone YcdY | - |
| ECSP_RS06890 | 1403309..1403800 | + | 492 | WP_001301587.1 | DUF1097 domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Genomic island | - | csgG / csgF / csgE / csgD / csgB / csgA / csgC | 1397086..1409368 | 12282 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 14117.17 Da Isoelectric Point: 7.7761
>T24183 WP_000854914.1 NC_013008:1398430-1398804 [Escherichia coli O157:H7 str. TW14359]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
Download Length: 375 bp
>T24183 NC_013008:1398430-1398804 [Escherichia coli O157:H7 str. TW14359]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13718.51 Da Isoelectric Point: 6.6249
>AT24183 WP_001280954.1 NC_013008:1398009-1398383 [Escherichia coli O157:H7 str. TW14359]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
Download Length: 375 bp
>AT24183 NC_013008:1398009-1398383 [Escherichia coli O157:H7 str. TW14359]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U9LXR5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0B0VS41 |