Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1398009..1398804 Replicon chromosome
Accession NC_013008
Organism Escherichia coli O157:H7 str. TW14359

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag ECSP_RS06850 Protein ID WP_000854914.1
Coordinates 1398430..1398804 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag ECSP_RS06845 Protein ID WP_001280954.1
Coordinates 1398009..1398383 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECSP_RS06805 1393364..1394269 + 906 WP_000203541.1 chemotaxis protein -
ECSP_RS06810 1394266..1395336 + 1071 WP_000102669.1 patatin-like phospholipase family protein -
ECSP_RS06820 1395676..1396494 + 819 WP_001234682.1 DUF945 domain-containing protein -
ECSP_RS06825 1396585..1397070 + 486 WP_000214398.1 antirestriction protein -
ECSP_RS06830 1397086..1397562 + 477 WP_001186738.1 RadC family protein -
ECSP_RS06835 1397625..1397846 + 222 WP_001220314.1 DUF987 domain-containing protein -
ECSP_RS06845 1398009..1398383 + 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
ECSP_RS06850 1398430..1398804 + 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
ECSP_RS06855 1398801..1399292 + 492 WP_000976853.1 hypothetical protein -
ECSP_RS06860 1399304..1399501 + 198 WP_000839282.1 DUF957 domain-containing protein -
ECSP_RS06865 1399586..1400428 + 843 WP_001280481.1 DUF4942 domain-containing protein -
ECSP_RS06875 1400899..1401837 + 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
ECSP_RS06880 1401892..1402629 + 738 WP_000283664.1 zinc-binding phosphatase -
ECSP_RS06885 1402653..1403207 + 555 WP_001001917.1 molecular chaperone YcdY -
ECSP_RS06890 1403309..1403800 + 492 WP_001301587.1 DUF1097 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - csgG / csgF / csgE / csgD / csgB / csgA / csgC 1397086..1409368 12282


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T24183 WP_000854914.1 NC_013008:1398430-1398804 [Escherichia coli O157:H7 str. TW14359]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T24183 NC_013008:1398430-1398804 [Escherichia coli O157:H7 str. TW14359]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT24183 WP_001280954.1 NC_013008:1398009-1398383 [Escherichia coli O157:H7 str. TW14359]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT24183 NC_013008:1398009-1398383 [Escherichia coli O157:H7 str. TW14359]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References