Detailed information of TA system TA024181

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1179559..1179784
• Replicon: chromosome
• Accession: NC_013008
• Organism: Escherichia coli O157:H7 str. TW14359

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ECSP_RS05645
• Protein ID: WP_000813263.1
• Coordinates: 1179629..1179784 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1179559..1179617 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSP_RS05620	1174828..1175925	+	1098	WP_001475117.1	hypothetical protein	-
ECSP_RS05625	1175932..1176678	+	747	WP_000788745.1	ATP-binding protein	-
ECSP_RS05630	1176700..1177470	+	771	WP_000450992.1	DUF1627 domain-containing protein	-
ECSP_RS05635	1177486..1177899	+	414	WP_001151233.1	DUF977 family protein	-
ECSP_RS05640	1178251..1179024	-	774	WP_000160654.1	alpha/beta hydrolase	-
-	1179559..1179617	-	59	-	-	Antitoxin
ECSP_RS05645	1179629..1179784	+	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
ECSP_RS31850	1179952..1180230	+	279	WP_001341388.1	hypothetical protein	-
ECSP_RS05655	1180232..1181281	+	1050	WP_001265172.1	DUF968 domain-containing protein	-
ECSP_RS05660	1181294..1181665	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ECSP_RS05665	1181655..1182026	+	372	WP_000090264.1	antitermination protein	-
ECSP_RS05670	1182178..1182996	+	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ECSP_RS05675	1183283..1183479	+	197	Protein_1068	TrmB family transcriptional regulator	-
ECSP_RS05680	1183617..1184330	+	714	WP_000261909.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T24181 WP_000813263.1 NC_013008:1179629-1179784 [Escherichia coli O157:H7 str. TW14359]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T24181 NC_013008:1179629-1179784 [Escherichia coli O157:H7 str. TW14359]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT24181 NC_013008:c1179617-1179559 [Escherichia coli O157:H7 str. TW14359]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin