Detailed information of TA system TA241800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 55680..56407
• Replicon: plasmid p21080937_2
• Accession: NZ_CP095255
• Organism: Klebsiella pneumoniae strain 21080937

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: MU320_RS27585
• Protein ID: WP_011251285.1
• Coordinates: 55680..55991 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MU320_RS27590
• Protein ID: WP_011251286.1
• Coordinates: 55988..56407 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU320_RS27545 (MU320_27545)	50691..51185	+	495	WP_011251274.1	hypothetical protein	-
MU320_RS27550 (MU320_27550)	51191..51631	+	441	WP_011251275.1	hypothetical protein	-
MU320_RS27555 (MU320_27555)	52056..53012	-	957	WP_011251280.1	DsbA family protein	-
MU320_RS27560 (MU320_27560)	53072..53413	-	342	WP_011251281.1	hypothetical protein	-
MU320_RS27565 (MU320_27565)	53427..53738	-	312	WP_011251282.1	hypothetical protein	-
MU320_RS27570 (MU320_27570)	53755..54204	-	450	WP_011251283.1	thioredoxin fold domain-containing protein	-
MU320_RS27580 (MU320_27580)	55038..55475	+	438	Protein_66	DDE-type integrase/transposase/recombinase	-
MU320_RS27585 (MU320_27585)	55680..55991	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
MU320_RS27590 (MU320_27590)	55988..56407	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
MU320_RS27595 (MU320_27595)	56554..57522	+	969	WP_074428168.1	IS5 family transposase	-
MU320_RS27600 (MU320_27600)	57594..57959	-	366	WP_048333448.1	hypothetical protein	-
MU320_RS27605 (MU320_27605)	57973..58761	-	789	WP_040217257.1	hypothetical protein	-
MU320_RS27610 (MU320_27610)	58782..59402	-	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
MU320_RS27615 (MU320_27615)	59821..60456	+	636	WP_223171879.1	hypothetical protein	-
MU320_RS27620 (MU320_27620)	60769..61239	+	471	WP_048333570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1	rmpA / rmpA / iroN / iroD / iroC / iroB	1..132724	132724
	-	inside	IScluster/Tn	-	-	47687..67380	19693

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T241800 WP_011251285.1 NZ_CP095255:55680-55991 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T241800 NZ_OU016037:c189453-189181 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT241800 WP_011251286.1 NZ_CP095255:55988-56407 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT241800 NZ_OU016037:c189716-189453 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1