Detailed information of TA system TA241771

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 45822..46549
• Replicon: plasmid p21080534_1
• Accession: NZ_CP095248
• Organism: Klebsiella pneumoniae strain 21080534

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: MU311_RS27100
• Protein ID: WP_011251285.1
• Coordinates: 45822..46133 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MU311_RS27105
• Protein ID: WP_011251286.1
• Coordinates: 46130..46549 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU311_RS27060 (40833)	40833..41327	+	495	WP_011251274.1	hypothetical protein	-
MU311_RS27065 (41333)	41333..41773	+	441	WP_011251275.1	hypothetical protein	-
MU311_RS27070 (42198)	42198..43154	-	957	WP_011251280.1	DsbA family protein	-
MU311_RS27075 (43214)	43214..43555	-	342	WP_011251281.1	hypothetical protein	-
MU311_RS27080 (43569)	43569..43880	-	312	WP_011251282.1	hypothetical protein	-
MU311_RS27085 (43897)	43897..44346	-	450	WP_011251283.1	thioredoxin fold domain-containing protein	-
MU311_RS27095 (45180)	45180..45617	+	438	Protein_52	DDE-type integrase/transposase/recombinase	-
MU311_RS27100 (45822)	45822..46133	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
MU311_RS27105 (46130)	46130..46549	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
MU311_RS27115 (47517)	47517..48440	+	924	WP_108970992.1	IS5 family transposase	-
MU311_RS27120 (48512)	48512..48877	-	366	WP_048333448.1	hypothetical protein	-
MU311_RS27125 (48891)	48891..49679	-	789	WP_040217257.1	hypothetical protein	-
MU311_RS27130 (49700)	49700..50320	-	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
MU311_RS27135 (50739)	50739..51374	+	636	WP_223171879.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaSHV-12	rmpA2 / iutA / iucD / iucC / iucB / iucA / iucA	1..210914	210914
	-	inside	IScluster/Tn	-	-	38913..55165	16252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T241771 WP_011251285.1 NZ_CP095248:45822-46133 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T241771 NZ_OU015345:185042-185308 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT241771 WP_011251286.1 NZ_CP095248:46130-46549 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT241771 NZ_OU015345:184779-185042 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1