Detailed information of TA system TA241750

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 128553..128806
• Replicon: plasmid p21080237_2
• Accession: NZ_CP095242
• Organism: Klebsiella pneumoniae strain 21080237

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: MU300_RS28360
• Protein ID: WP_001312851.1
• Coordinates: 128657..128806 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	128553..128612 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU300_RS28320 (123935)	123935..124279	-	345	Protein_157	IS6-like element IS26 family transposase	-
MU300_RS28325 (124331)	124331..125035	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
MU300_RS28330 (125060)	125060..125260	+	201	WP_072354025.1	hypothetical protein	-
MU300_RS28335 (125280)	125280..126026	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
MU300_RS28340 (126081)	126081..126641	+	561	WP_000139328.1	fertility inhibition protein FinO	-
MU300_RS28345 (126773)	126773..126973	+	201	WP_015059022.1	hypothetical protein	-
MU300_RS28350 (127359)	127359..127958	+	600	WP_032083981.1	PIN domain-containing protein	-
MU300_RS28355 (128020)	128020..128352	+	333	WP_152916585.1	hypothetical protein	-
- (128553)	128553..128612	-	60	NuclAT_1	-	Antitoxin
- (128553)	128553..128612	-	60	NuclAT_1	-	Antitoxin
- (128553)	128553..128612	-	60	NuclAT_1	-	Antitoxin
- (128553)	128553..128612	-	60	NuclAT_1	-	Antitoxin
MU300_RS28360 (128657)	128657..128806	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
MU300_RS28365 (129090)	129090..129338	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..129649	129649

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T241750 WP_001312851.1 NZ_CP095242:128657-128806 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T241750 NZ_OU015340:3594237-3594605 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT241750 NZ_CP095242:c128612-128553 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin